猪瘟病毒兔化弱毒株cDNA片段的克隆及序列分析  

MOLECULAR CLONE AND SEQUENCE ANALYSIS OF cDNA FRAGMENTS OF HOG CHOLERA VIRUS STRAIN C 

作　　者：李红卫[1] 刘湘涛 李小兵[1] 韩雪清 涂长春[1] 殷震[1] 

机构地区：[1]长春农牧大学,长春130062 [2]兰州兽医研究所,兰州730046

出　　处：《微生物学报》1999年第6期554-558,共5页Acta Microbiologica Sinica

基　　金：国家攀登计划B 类项目资助课题

摘　　要：The 5′ nonencoding region, p23 and p14 encoding region and E1 gene of hog cholera virus (HCV) strain C were amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested or half\_nested PCR. The PCR products were cloned into pGEM\|T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device; based on the incorporation of fluoresect labelled dideoxynuclotide teminators. The obtained sequences on 5′ noncoding region and part of p23 and p14 encoding region were compared with HCV strains Shimen and C sequenced in Moormann’s lab. The result showed that the homology between HCV strains C sequenced in this report and in Moormann’s lab was 99.19%, and the homology between HCV strains Shimen, the standard virulent HCV strain in China, and C sequenced in this report was 94.69%. It was also discovered that the base C at 244 of the genome of HCV strains Shimen and C sequenced in this report was absent at the genome of C strain sequenced in Moormann’s lab et al.The 5′ nonencoding region, p23 and p14 encoding region and E1 gene of hog cholera virus (HCV) strain C were amplified from total RNA extracted from HCV strain C infected rabbit spleen by reverse transcription and nested or half\_nested PCR. The PCR products were cloned into pGEM\|T vector. Nucleotide sequencing was performed using an ABI PRISM sequencing device; based on the incorporation of fluoresect labelled dideoxynuclotide teminators. The obtained sequences on 5′ noncoding region and part of p23 and p14 encoding region were compared with HCV strains Shimen and C sequenced in Moormann's lab. The result showed that the homology between HCV strains C sequenced in this report and in Moormann's lab was 99.19%, and the homology between HCV strains Shimen, the standard virulent HCV strain in China, and C sequenced in this report was 94.69%. It was also discovered that the base C at 244 of the genome of HCV strains Shimen and C sequenced in this report was absent at the genome of C strain sequenced in Moormann's lab et al.

关 键 词：cDNA 克隆 序列分析 HCLV 猪瘟疫苗 

分 类 号：S858.285.3[农业科学—临床兽医学] Q78[农业科学—兽医学]