嗜碱菌A4-10碱性纤维素酶产生条件及性质的初步研究  

STUDIES ON PRODUCTION AND PROPERTIES OF ALKLINE CELLULASE FROM ALKALOPHILIC BACTERIUM A4-10

作　　者：迪丽拜尔.托乎提 田新玉[2] 周培瑾[2] 

机构地区：[1]新疆师范大学生物系,乌鲁木齐830054 [2]中国科学院微生物研究所,北京100080

出　　处：《应用与环境生物学报》1999年第6期632-634,共3页Chinese Journal of Applied and Environmental Biology

摘　　要：从４８ 份土壤和天然碱湖样品分离出能产生胞外碱性纤维素酶的嗜碱细菌１３５ 株，其中７ 株菌产酶活力大于３ Ｕ／ｍＬ，菌株Ａ４１０ 经条件试验后酶活力达２１ Ｕ／ｍＬ，该菌产生碱性纤维素酶最适培养温度为３４ ℃，最适碳源为ＣＭＣＮａ，最适氮源为复合蛋白胨和酵母粉，该酶的最适反应温度５５ ℃，最适反应ｐＨ９．０ ．strains of alkalophilic bacteria were isolated from 48 samples of soil and soda lakes, and all of them were able to produce alkaline cellulase. The enzyme activities of 7 strains were higher than 3 U/mL, and the strain A4 10 showed the maximum activity that was 21 U/mL. The maximum growth of the alkalophilic bacteria was observed at pH 9.0 with the optimum temperature of 34℃ for enzyme production, the optimum carbon source being CMC Na and the optimum nitrogen source being peptone or yeast extract (DIFCO).The optimum temperature and pH for enzymatic catalysis were 55℃ and 9.0,respectively.

关 键 词：嗜碱细菌 碱性纤维素酶 产酶条件 酶学性质 

分 类 号：TQ925.9[轻工技术与工程—发酵工程] Q556.203[生物学—生物化学]