Fluoro-Jade C揭示小鼠匹罗卡品模型中杏仁核神经变性  

Fluoro-Jade C can specifically stain the degenerative neurons in the amygdaloid complex of pilocarpine-treated mice

作　　者：王莲[1] 魏玲[1] 魏伟[1] 

机构地区：[1]成都军区昆明总医院地干病房,云南昆明650032

出　　处：《中国现代医学杂志》2011年第24期2974-2976,2988,共4页China Journal of Modern Medicine

摘　　要：目的应用Fluoro-Jade C(FJC)染色方法在小鼠匹罗卡品癫痫模型中探测杏仁核结构中神经元的变性情况,以了解杏仁核结构在慢性颞叶癫痫发生中的病理变化和癫痫反复发作的神经基础。方法雄性昆明小鼠6只(对照组3只,匹罗卡品处理组3只)。在癫痫持续状态后12 h处死处理组小鼠。在杏仁核水平切制冠状切片,行FJC染色,在荧光显微镜下观察FJC阳性细胞的形态和在杏仁核中的整体分布情况。结果在处理组,FJC染色的脑切片上可以很清楚地看到呈亮黄绿色荧光的FJC阳性细胞,呈神经元形态,胞体和突起均清晰显示。杏仁核出现大量FJC阳性细胞,而对照组未见。结论该研究在小鼠匹罗卡品癫痫模型中运用FJC染色技术显示杏仁核中发生了大量神经元变性,有利于更好地理解颞叶癫痫中中枢神经系统所发生的病理变化和自发反复发作的癫痫机制。【Abjective】 To detect degenerative neurons by Fluoro-Jade C（FJC） that may occur in the amygdaloid complex of pilocarpine-treated mice and to understand the neural basis of amygdaloid complex in long-term brain pathological changes and recurrent seizure mechanism of chronic epilepsy.【Methods】 Male adult mice（Kunming stain,n =6） were divided into pilocarpine-treated group（n =3） and controls（n =3）.Pilocarpine-treated mice were sacrificed at 12 h after status epilepticus.Brain coronal sections in the amygdaloid complex levels were subject to FJC staining.Finally,FJC-stained brain sections were examined under an epifluorescence microscope to identify the shape and overall distribution of the Fluoro-Jade C positive cells in the amygdaloid complex.【Results】 The FJC-positive staining cells showed a bright green color in the somas and fine processes with neuronal profiles.Numerous degenerating cells could be seen in the amygdaloid complex.They were not detected in control animals.【Conclusion】 This study successfully applied FJC staining to demonstrate massive neurodegeneration in the amygdaloid complex,which will benefit further understanding of brain pathological changes and recurrent seizure mechanism in temporal lobe epilepsy.

关 键 词：FLUORO-JADE C 癫痫 匹罗卡品 神经变性 

分 类 号：R742.1[医药卫生—神经病学与精神病学]