Lupeol及其衍生/修饰物对肝癌细胞增殖影响的研究  

Effects of Lupeol and its modified compounds on cell proliferation of hepatocellular carcinoma cell lines

作　　者：刘芬[1] 何艳[1] 李云森[1] 朱永明[2] 刘海燕[1] 

机构地区：[1]苏州大学生物医学研究院肿瘤细胞与分子免疫实验室,江苏苏州215123 [2]苏州大学医学部药学院,江苏苏州215123

出　　处：《现代肿瘤医学》2011年第12期2393-2396,共4页Journal of Modern Oncology

基　　金：苏州市重点实验室资助项目(编号:SWG0904;SS201032)

摘　　要：目的:探讨lupeol及其结构改造后衍生/修饰物对肝癌细胞HepG2和SMMC7721细胞增殖的作用。方法:对lupeol进行化学结构修饰,然后应用MTT方法检测lupeol及其衍生/修饰物对HepG2和SMMC7721细胞增殖的作用。结果:MTT数据显示,lupeol及其衍生/修饰物均能抑制HepG2和SMMC7721细胞增殖,并且具有浓度依赖性。Lupeol、H1、T1和T2的IC50分别为40μmol/L-50μmol/L、约120μmol/L、约50μmol/L和70μmol/L-80μmol/L。结论:lupeol结构改造后,并没有提高其对肝细胞肝癌细胞增殖的抑制作用。Objective：To investigate the effects of lupeol and its modified compounds on proliferation of hepatocellular carcinoma cells HepG2 and SMMC7721.Methods： The structure of lupeol was modified.Then cell viability of HepG2 and SMMC7721 was measured by MTT assay after treatment with increasing concentration of lupeol or its modified compounds.Results： MTT results showed that lupeol and its modified compounds inhibited the proliferation of HepG2 and SMMC7721 cells in a concentration dependent manner.IC50 of lupeol,H1,T1 and T2 were 40 μmol/L-50 μmol/L,about 120 μmol/L,about 50 μmol/L and 70 μmol/L-80 μmol/L,respectively.Conclusion： Modification of lupeol performed in the current study did not improve its therapeutic effects in hepatocellular carcinoma.

关 键 词：肝细胞肝癌细胞系 LUPEOL 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]