EGCG对LoVo和SW480结肠癌细胞株的作用及对Notch1与Notch2基因表达的影响  

Effect of epigallocatechin-3-gallate on Notch1 and Notch2 expression in human colorectal cancer cell lines SW480 and LoVo

作　　者：张春霞[1] 王水明[2] 何勇山[1] 周阿成[1] 金黑鹰[2] 

机构地区：[1]南京中医药大学研究生院,江苏省南京市210029 [2]全国肛肠医疗中心南京中医药大学第三附属医院,江苏省南京市210001

出　　处：《世界华人消化杂志》2011年第29期3023-3027,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30572447;No.30973837;江苏省自然科学基金资助项目;No.BK2007009~~

摘　　要：目的:探讨绿茶提取物表没食子儿茶素没食子酸酯(EGCG)对结肠癌细胞株LoVo细胞和SW480细胞增殖的抑制作用,研究其对Notch1与Notch2的基因表达的影响.方法:体外培养LoVo细胞和SW480细胞,采用不同浓度的EGCG(10、20、35mg/L)对其进行干预,MTT法检测EGCG对LoVo细胞和SW480细胞增殖的抑制作用;用流式细胞仪检测EGCG对LoVo细胞和SW480细胞细胞凋亡和细胞周期的影响.实时荧光定量PCR检测EGCG干预后的LoVo细胞和SW480细胞的Notch1与Notch2基因的表达情况.结果:MTT法检测EGCG对LoVo细胞和SW480细胞增殖均具有抑制作用,且呈浓度依赖性,不具有时间依赖性.流式细胞仪检测EGCG能增强LoVo细胞和SW480细胞的凋亡率,且剂量与凋亡率呈正相关.EGCG能将SW480细胞和LoVo细胞周期阻滞在G0/G1期,阻碍其向S期转换,抑制其细胞增殖.实时荧光定量PCR检测EGCG能下调两株细胞Notch2的基因表达,而SW480细胞的Notch1基因表达有下调的趋势,LoVo细胞的Notch1基因表达有上调的趋势,但差异均无显著意义.结论:EGCG对体外培养的LoVo细胞和SW480细胞的增殖有明显抑制作用,且能诱导细胞凋亡和影响细胞周期,其作用机制可能与下调Notch2的基因表达及激活Notch信号转导途径有关,但是EGCG对Notch1基因表达的影响尚需要做进一步探讨.AIM： To investigate the inhibitory effect of epigallocatechin-3-gallate （EGCG） on the proliferation of human colorectal cancer cell lines LoVo and SW480 and to explore the possible mechanisms involved. METHODS： Cultured LoVo and SW480 cells were used to examine the impact of different concentrations of EGCG （10, 20 and 35 mg/L） on cell proliferation by MTT assay and on cell apoptosis and cell cycle progression by flow cytometry. The expressing of Notch1 and Notch2 in cultured LoVo and SW480 cells was detected by real-time PCR. RESULTS： EGCG inhibited the proliferation of LoVo cells and SW480 cells in a dose-dependent, time-independent manner. EGCG enhanced the apoptosis of LoVo cells and SW480 cells and blocked the cell cycle at G0/G1 phase. Moreover, EGCG down-regulated Notch2 gene expression but had no significant impact on Notch1 gene expression in LoVo cells and SW480 cells. CONCLUSION： EGCG can significantly inhibit the proliferation of SW480 and LoVo cells by altering cell cycle and inducing cell apoptosis via mechanisms associated with down-regulating Notch2 gene expression.

关 键 词：表没食子儿茶素没食子酸酯 LOVO细胞 SW480细胞 NOTCH2 NOTCH1 

分 类 号：R735.3[医药卫生—肿瘤]