不同剂量致敏原对幼年大鼠哮喘的影响  

Effect of different doses of sensibiligen on childhood rats of atopic asthma

作　　者：刘佩意[1] 蒋卓勤[1] 陈相[1] 冷亮[1] 马立丽 

机构地区：[1]中山大学公共卫生学院营养学系,广东广州510080

出　　处：《中国现代医学杂志》2011年第31期3851-3855,共5页China Journal of Modern Medicine

摘　　要：目的利用卵清蛋白(OVA)致敏刚断乳的大鼠,建立幼年大鼠哮喘模型,探讨不同剂量的致敏原对幼年大鼠哮喘气道过敏性炎症的影响。方法用低、中、高剂量卵清蛋白致敏大鼠后再雾化吸入同一致敏原从而诱发大鼠哮喘发作,分别观察各组大鼠肺组织病理切片、支气管肺泡灌洗液(BALF)中细胞计数、双抗体夹心酶联免疫吸附试验法检测BALF中OVA特异性IgE(OVA-IgE)和外周血白细胞介素(IL-4)的水平。结果低、中和高剂量组的大鼠较正常对照组均出现气道过敏的异常症状,但高剂量组有明显的腹式呼吸和呼吸短促,且高剂量组大鼠的肺组织病理显示气道炎症较低、中剂量组明显严重,3个致敏组的BALF中细胞总数较对照组均有不同程度增高,高剂量组增高显著(P<0.05)。高剂量组大鼠血清中IL-4和BALF中OVA-IgE较对照组和低、中剂量组均明显增高(P<0.05),中剂量组BALF中OVA-IgE较对照组增高显著(P<0.05)。结论幼年大鼠哮喘模型中的致敏原剂量的大小与哮喘气道过敏性炎症的程度有关,致敏原剂量越大,气道过敏性炎症越明显。【Objective】 Sensitize the Wistar Rats in childhood with ovalbumin,and set a rat model in childhood of atopic asthma,to investigate the effect of different doses of sensitinogen on airway allergic inflammation in the childhood rat model.【Methods】 The asthma model of Wistar rats in childhood were prepared by ovalbumin（OVA） and alminum hydroxide（Alum） with a low,a middle and a high dose respectively.The pathologic changes of lung tissue,the total cell count in bronchoalveolar lavage fluid（BALF） were observed.The concentrations of IL-4 in serum and OVA-IgE in BALF were measured by using sandwich ELISA.【Results】 Low-dose,middle-dose and high-dose,the three sensitized model groups suffered from asthma abnormal symptoms compared with control group,and the high dose group had a significant abdominal breathing and shortness of breath.The inflammation in high dose group was more severe than both low dose group and middle dose group.Total cell count of BALF in the three sensitized groups were increased,to varying degree,compared with control group,most significantly in high dose group（P 0.05）.The serum IL-4 and BALF OVA-specific IgE were augmented significantly in high dose group compared with control group,low dose group and middle dose group（P 0.05）,and the BALF OVA-specific IgE was higher than control group as well（P 0.05）.【Conclusion】 The doses of antigen in a childhood rat model of atopic asthma are related with the extent of airway allergic inflammation.The more antigen it is sensitized,the severer airway allergic inflammation is.

关 键 词：气道过敏性炎症 幼年大鼠 哮喘 

分 类 号：R-332[医药卫生]