响应面分析法优化超临界二氧化碳萃取橘皮中类柠檬苦素工艺  

Application of Response Surface Methodology to Optimize Extraction Process for Nomilin from Orange Peel with Supercritical Carbon Dioxide

作　　者：曾祥燕[1] 赵良忠[1] 

机构地区：[1]邵阳学院生物与化学工程系,湖南邵阳422200

出　　处：《食品科学》2011年第22期65-69,共5页Food Science

基　　金：湖南省教育厅一般研究项目(10C1190)

摘　　要：研究利用超临界二氧化碳萃取橘皮中类柠檬苦素工艺,考察萃取时间、萃取温度、萃取压力和夹带剂用量对类柠檬苦素提取量的影响,采用响应面试验设计,以类柠檬苦素提取量为响应值进行考察。结果表明:4个因素对类柠檬苦素提取量的影响大小次序为时间>萃取压力>萃取温度>夹带剂用量;通过对模型进行分析,得出提取类柠檬苦素的最佳工艺条件为萃取时间2h、萃取温度50℃、萃取压力30MPa、夹带剂用量1.51mL/g,在此条件下,类柠檬苦素的提取量为54.26mg/kg,而实际提取量为53.87mg/kg,回归方程的预测值和试验值无显著性差异。所得回归模型拟合情况良好,能很好地反应实际情况。In the extraction of nomilin from orange peel with supercritical CO2,four operating parameters including time,temperature,pressure and entrainer（anhydrous ethanol） amount that affect extraction yield of nomilin were investigated using response surface methodology（RSM）.A mathematical model describing the relationship between them was set up.The operating parameters could be ranked in decreasing order of influence as follows： time,pressure,temperature and entrainer amount.Response surface analysis of the developed model showed that the optimal extraction conditions were 2 h,50 ℃,30 MPa and 1.51 mL/g entrainer amount.Under the conditions,the predicted and experimental extraction yields of nomilin were 54.26 mg/kg and 53.87 mg/kg,respectively,showing no significant difference.Moreover,the experimental data were well fitted with the developed model.

关 键 词：类柠檬苦素 工艺 优化 响应面 超临界二氧化碳 

分 类 号：TS201.1[轻工技术与工程—食品科学]