高效液相色谱-示差折光法检测紫胶桐酸  

Determination of Aleuritic Acid by High Performance Liquid Chromatography with Differential Refractive Index Detector

作　　者：刘世平[1,2] 张弘[2] 周梅村[1] 郑华[2] 李凯[1,2] 郭元亨[2] 

机构地区：[1]昆明理工大学化学工程学院,云南昆明650224 [2]中国林业科学研究院资源昆虫研究所,云南昆明650224

出　　处：《食品科学》2011年第22期260-263,共4页Food Science

基　　金：国家林业局"948"项目(2008-4-78);科技部农业科技成果转化资金项目(2010GB24320619);国家林业局林业科技成果推广项目([2010]11)

摘　　要：采用高效液相色谱(high performance liquid chromatography,HPLC)法,利用示差折光检测器(refractiveindex detector,RID)测定紫胶桐酸含量。色谱柱为Agilent ZORBAX SB-C18(4.6mm×250mm,5μm),以V(甲醇):V(水)=60:4 0(0.1%三氟乙酸)作为流动相,流速1 m L/m i n,进样体积1 0μL,柱温3 0℃。结果表明:紫胶桐酸在0.01～1.0mg/mL呈良好的线性关系,R2=0.9994;最低检测限为0.008mg/mL;精密度RSD值为0.86%;平均加标回收率为100.23%,RSD值为0.65%。采用HPLC-RID测定紫胶桐酸的含量,操作简单,结果准确,稳定性和可信度好。In this paper,we present a high performance liquid chromatography（HPLC） with differential refractive index detector（RID） method to determine aleuritic acid.Ten mL of the analyte was chromatographically separated on an Agilent ZORBAX SB-C18 column（4.6 mm × 250 mm,5μm） using a mobile phase consisting of a mixture of methanol and water（60：40,containing 0.1% trifluoroacetic acid） at a flow rate of 1 mL/min.The column temperature was set as 30 ℃.Aleuritic acid showed a good linear relationship over the concentration range of 0.01—1.0 mg/mL with a correlation coefficient of 0.9994.The limit of detection was 0.008 mg/mL.The precision RSD was 0.86%（n =9）.The average spike recovery rate was 100.23% with a RSD of 0.65%（n = 9）.The method was easy to operate,simple,accurate,stable and reliable.

关 键 词：紫胶桐酸 高效液相色谱(HPLC) 示差折光检测器(RID) 

分 类 号：TS207.3[轻工技术与工程—食品科学]