Hedgehog信号通路中SHH蛋白及其下游转录因子GLI1在Peutz-Jeghers综合征中的表达及意义  

Expression of SHH protein and its downstream transcriptional factor GLI1 in Hedgehog signal pathway in Peutz-Jeghers syndrome

作　　者：苏娟[1] 吴保平[1] 徐晓平[1] 李冉[1] 

机构地区：[1]南方医科大学南方医院消化内科,广州510515

出　　处：《第二军医大学学报》2011年第12期1306-1309,共4页Academic Journal of Second Military Medical University

基　　金：广东省自然科学基金(9151051501000069)~~

摘　　要：目的检测Hedgehog信号通路中SHH蛋白及其下游转录因子GLI1蛋白在人Peutz-Jeghers综合征(PJS)息肉中的表达,探讨其在PJS发生及恶变中的作用。方法采用免疫组织化学方法检测PJS息肉(20例)、正常肠黏膜组织(20例)、大肠腺瘤性息肉(25例)、大肠腺癌(25例)组织中SHH和GLI1蛋白的表达和分布,并比较表达程度的差异。结果免疫组织化学检测SHH蛋白和GLI1蛋白的阳性着色主要分布于胞膜和胞质,腺癌中GLI1蛋白的胞质和核染色增加。SHH蛋白和GLI1蛋白在正常肠黏膜组织、PJS息肉组织、腺瘤组织及腺癌组织中的阳性表达率和表达强度依次增高,差异具有统计学意义(Kruskal-Wallis H test,P<0.001)。SHH与GLI1的蛋白表达在PJS中经Spearman等级相关分析呈正相关(rp=0.503,P=0.024)。结论 SHH/GLI1在正常肠黏膜组织、PJS息肉组织、腺瘤组织及腺癌组织中均有表达,且表达水平依次增高,提示SHH-GLI1信号通路可能与PJS息肉的发生及恶变有关。Objective To detect the protein expression of SHH and its downstream transcriptional factor GLI1 in Peutz-Jeghers syndrome（PJS） and to investigate the roles of SHH and GLI1 in the development and carcinogenesis of PJS polyps.Methods Immunohistochemical（IHC） staining was employed to detect the expression and localization of SHH and GLI1 protein in 20 PJS polyp samples,25!adenomatous polyp tissues,25 colon adenocarcinoma samples,and 20 normal intestinal mucosal tissues.Results IHC revealed that SHH protein and GLI1 protein were mainly localized on cell membrane and in the cytoplasm,and the nuclear staining of GLI1 protein in the colon adenocarcinoma was increased.The expression of the two proteins gradually increased in order in the normal intestinal mucosa,PJS polyp,adenomatous polyp,and colon adenocarcinoma（P0.001）.Spearman rank correlation showed that SHH expression was significantly correlated with GLI1 expression in PJS polyp（rp=0.503,P=0.024）.Conclusion SHH-GLI1 is present in the tissues of normal intestinal mucosa,PJS polyp,adenomatous polyp,and colon adenocarcinoma,and the expression has an increasing tendency in the above tissues,indicating SHH-GLI1 signal pathway may be associated with the development and carcinogenesis of PJS polyp.

关 键 词：PEUTZ-JEGHERS综合征 SHH GLI1 结直肠肿瘤 腺瘤性息肉 腺癌 

分 类 号：R735.3[医药卫生—肿瘤]