生存素反义寡核苷酸对血管瘤血管内皮细胞增殖及Caspase-3活性影响研究  

Effect of Survivin ASODN on proliferation of vascular endothelial cells of hemangioma and Caspase-3 activity

作　　者：谭海松[1] 房艳[1] 王绪凯[1] 

机构地区：[1]中国医科大学口腔医学院口腔颌面外科,沈阳110002

出　　处：《中国实用口腔科杂志》2011年第11期656-658,共3页Chinese Journal of Practical Stomatology

摘　　要：目的观察生存素(Survivin)反义寡核苷酸(ASODN)对人血管瘤血管内皮细胞凋亡的影响,并初步探讨其作用机制。方法实验于2007年12月至2010年2月完成。以靶向Survivin基因中与Caspase-3结合的部位为模板,设计合成ASODN并通过脂质体将其转染至人血管瘤血管内皮细胞中。MTT观察Survivin ASODN对血管瘤血管内皮细胞增殖的影响;分光光度法检测Caspase-3酶活性。结果 Survivin ASODN可以显著抑制血管瘤血管内皮细胞增殖,而且抑制程度与Survivin ASODN浓度成正比。Survivin ASODN作用于血管瘤血管内皮细胞,Caspase-3酶活性呈时间、剂量依赖性升高。结论以靶向Survivin基因中与Caspase-3结合的部位为模板设计合成的ASODN可显著抑制人血管瘤血管内皮细胞增殖,诱导细胞凋亡,其机制与提高Caspase-3酶活性有关。Objective To observe the effect of Survivin antisense oligodeoxynucleotid （ASODN） on the apoptosis of vascular endothelial cells of haemangioma and to explore its mechanism. Methods Targeting Survivin gene in Caspase-3 binding sites to design and synthesis of ASODN, different concentrations of Survivin ASODN and control sequence （scrambled ODN） were transferred into the vascular endothelial ceils of haemangioma by alipofectin reagent. The Thiazolyl blue（MTT）assay was used to measure the growth inhibitory rate. Activity of Caspase-3 enzyme was analyzed by spectrophotometry. Results After 44 hours of Survivin ASODN treatment, distinct morphologic changes characteristic of cell apoptosis were significant. The Caspase-3 activity in vascular endothelial cells of haemangioma treated with Survivin ASODN increased significantly. Conclusion Survivin ASODN can inhibit proliferation, induce apoptosis of vascular endothelial cells of haemangioma;the mechanism may be related to activating Caspase-3.

关 键 词：生存素 反义寡核苷酸 CASPASE-3 

分 类 号：R78[医药卫生—口腔医学]