广西眼镜蛇毒中Natrin蛋白的分离及纯化  

PURIFICATION OF NATRIN FROM GUANGXI COBRA VENOM

作　　者：蒋兴明[1] 焦杨[1] 宋慧[1] 马兴才 周燕[1] 周元聪[3] 舒雨雁[4] 

机构地区：[1]广西医科大学药学院药理学教研室,南宁530021 [2]广西南宁市第七人民医院,南宁530022 [3]中国科学院上海生化与细胞研究所,上海200031 [4]广西医科大学蛇毒研究所,南宁530021

出　　处：《广西医科大学学报》2011年第5期665-667,共3页Journal of Guangxi Medical University

基　　金：广西科学基金资助项目(No.桂科青0542043;No.桂科自0991127)

摘　　要：目的:从广西眼镜蛇毒中分离出Natrin蛋白。方法:广西眼镜蛇毒经Sephadex G75凝胶、CM Sepharose CL-6B离子交换层析及FPLC mono-S柱层析分离纯化出Natrin蛋白。结果:从广西眼镜蛇蛇毒中分离纯化得到的Natrin蛋白,经SDS-PAGE电泳测定其分子量为25ku,质谱测定其分子量为24.937ku,测得其N端的序列为NVDFNSESTRRKKKQKEIVD,C端的序列为NIKK。结论:从广西眼镜蛇毒中分离出纯的Natrin蛋白组分。Objective：To isolate and purify Natrin from Guangxi cobra venom.Methods：The Natrin from Guangxi cobra venom was sequentially purified by Sephasex G75gel,CM-sepharose CL-6B ion exchange chromatography and Mono S column chromatography on FPLC system.Results：The Natrin purified from Guangxi cobra venom tested by SDS-PAGE and proved its molecular weight was 24.937 ku and it＇s N-terminal acid sequence was NVDFNSESTRRKKKQKEIVD and C-terminal acid sequence was NIKK.Conclusion：Natrin can be obtained by gel filtration and ionexchange.

关 键 词：广西眼镜蛇毒 Natrin蛋白 分离纯化 

分 类 号：R-33[医药卫生]