双水相体系萃取盐酸小檗碱  

Extract Berberine Hydrochloride by Aqueous Two-phase System

作　　者：文赤夫[1,2] 向小奇[2] 曾卫湘[2] 吴婷[2] 田春莲[1] 

机构地区：[1]林产化工工程湖南省重点实验室,湖南张家界427000 [2]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《中药材》2011年第10期1608-1611,共4页Journal of Chinese Medicinal Materials

基　　金：林产化工工程湖南省重点实验室开放基金资助项目(JDZ201002)

摘　　要：目的:研究可用于盐酸小檗碱萃取的双水相体系。方法:先用3种双水相体系进行萃取试验,然后选择较好的一种体系进行单因素试验和正交试验。结果:向10 mL 95%乙醇和10 mL 2.2 mol/L硫酸铵溶液混合形成的双水相体系中加入质量分数为53.22%的盐酸小檗碱粗品600 mg,调节体系pH值至4,70℃水浴处理30 min,萃取率可达99.29%;将萃取液集中在一起,40℃下烘干,所得盐酸小檗碱的质量分数为88.43%。结论:该体系是适合于盐酸小檗碱萃取的双水相体系。Objective： To study the aqueous two-phase system which could be used for extraction of berberine hydrochloride. Methods ： Three aqueous two-phase systems were used for extraction experiment firstly, the best one was chosen from that, and then the single factor experiment and orthogonal experiment were carried out. Results ： 10 mL 95 % ethanol with 10 mL 2.2 mol/L ammonium sulfate could make a aqueous two-phase system, added 600 mg berberine hydrochloride whose purity was 53.22 % into it, regulated its pH to 4, then the system was put into water-bath heater （70℃ ） for 30 min, the extraction rate could reach 99.29 % ; Collected the extraction liquid, dried it under 40 ℃, the purity of berberine hydrochloride was 88. 43 %. Conclusion ： This system is a suitable aqueous two-phase system for extraction of berberiue hydrochloridc.

关 键 词：双水相 盐酸小檗碱 萃取 

分 类 号：R284.2[医药卫生—中药学]