高强度超声对人乳腺癌细胞中COX-2mRNA表达的影响  

Effect of hi2h intensity ultrasound on COX-2 mRNA expression in human breast cancer cells

作　　者：韩江涛[1] 孙广运[2] 黄建朴[1] 唐磊[1] 刘纪蓉[1] 谢明均[3] 

机构地区：[1]宜宾市第一人民医院超声科,宜宾644000 [2]宜宾市第一人民医院麻醉科,宜宾644000 [3]宜宾市第一人民医院普外科,宜宾644000

出　　处：《中华内分泌外科杂志》2011年第6期374-376,共3页Chinese Journal of Endocrine Surgery

基　　金：宜宾市重点科技项目（200703007）

摘　　要：目的探讨高强度超声对环氧化酶一2（COX一2）mRNA的表达及其影响乳腺癌MDA—MB-231细胞凋亡的作用机制。方法用高强度超声（50w／cm^2）干预体外培养的人乳腺癌MDA—MB-231细胞。RT—PCR检测干预前后MDA．MB一231的COX．2mRNA的相对水平，透射电镜观察细胞超微结构。结果随着高强度超声干预时间的增加，COX-2mRNA相对水平逐渐下调，电镜下可见明显的凋亡小体。干预时间增加到30s，部分细胞出现死亡的形态学改变。结论高强度超声干预在一定作用时间内促进乳腺癌MDA-MB一231细胞COX一2依赖性的细胞凋亡。Objective To study the mechanism of high intensity ultrasound （HIU） on COX-2 mRNA expression and apoptosis in breast cancer cell line MDA-MB-231 cells. Methods Human breast cancer cells MDA-MB-231 in vitro were exposed in HIU （50 w/em2）. RT-PCR was performed to detect the level of COX-2 mRNA before and after exposure. The uhrastruetural changes in apoptotie cells were examined by transmission e- lectron microscopy. Results The relative level of COX-2 mRNA decreased gradually along with the increase of exposure time and apoptotic bodies in MDA-MB-231 cells considerably increased under electron microscope. When exposure time was increased to 30 seconds, a few cells died. Conclusion HIU promotes apoptosis of breast cancer MDA-MB-231 cells in a COX-2 mRNA d^nended way.

关 键 词：高强度超声 环氧化酶一2 MRNA 乳腺癌 凋亡 

分 类 号：R737.9[医药卫生—肿瘤] R454.3[医药卫生—临床医学]