整合素阻断剂HM-3联合环磷酰胺应用的抗肿瘤作用  

Antitumor Effects of Integrin Inhibitor HM-3 and Cyclophosphamide in Mouse Melanoma

作　　者：任印玲[1] 刘振东[1] 潘丽[1] 沈鸿[1] 徐寒梅[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2011年第6期522-525,529,共5页Pharmaceutical Biotechnology

基　　金：国家新药创制科技重大专项(No.2009ZX09102-244);江苏省产学研项目资助

摘　　要：建立C57BL/6小鼠移植瘤模型,设置HM-3、环磷酰胺(CTX)、多西他赛(DOC)、联合给药及阴性对照组。治疗14 d后处死动物,剥取瘤组织称重,计算抑瘤率,并应用免疫组化法检测肿瘤组织的微血管密度(microvessel density,MVD)。实验结果证明联合给药同时给药组和联合给药先给CTX再给HM-3组与阴性对照组有极显著性差异(P<0.01);与阴性组相比较,联合组或单独HM-3组的MVD显著减少,统计学显示有显著性差异(P<0.05)。CTX与HM-3联合给药具有协同效应,并且治疗效果与二者给药顺序有相关性。The antitumor effects of HM-3 were evaluated in different sequences of combination with Cyclophosphamide （CTX） in B16F10 cells. The mouse melanoma cell line B16F10 cells were injected into C57BL/6 mice to make the melanoma model. Mice were randomly divided into seven groups：HM-3 treatment group, CTX treatment group, Docetaxel treatment group,concomitant treat- ment group, sequential treatment with the CTX followed by HM-3 group, sequential treatment with HM-3 followed by the CTX group and control group. Animals were sacrificed after 2 weeks. Tumor microvessel density （ MVD ） was determined by the immunohisto- chemistry method . HM-3 combined with CTX group and sequential treatment with the CTX followed by HM-3 group displayed a sig- nificant inhibitory effect compared with the control （P 〈 0.01 ）. The MVD was inhibited significantly by HM-3 in combination with CTX or HM-3 alone compared with the control （ P 〈 0.05 ）. Three different combination groups had different effects. The best effect was obtained when chemotherapy was followed by treatment with HM-3. The angiogenesis inhibitor HM-3 in combination with CTX has a synergistic effect and inhibitory activity against melanoma model, and the different antitumor effects are depending on the treatment schedule.

关 键 词：整合素阻断剂 HM-3 环磷酰胺 联合给药 黑色素瘤 

分 类 号：R73[医药卫生—肿瘤]