芥蓝菜薹不同节间芥子油苷组分及含量分析  

Composition and content of glucosinolates in different sections of bolting stems of Chinese kale

作　　者：腊贵晓[1] 刘国顺[1] 杨俞娟[2] 

机构地区：[1]河南农业大学烟草学院,河南郑州450002 [2]浙江大学环境与资源学院,浙江杭州310029

出　　处：《河南农业大学学报》2011年第6期651-656,667,共7页Journal of Henan Agricultural University

基　　金：国家重点基础研究发展计划资助项目(2007CB109305)

摘　　要：采用高效液相色谱及液相色谱-质谱联用法,以芥蓝(Brassica alboglabra L.)3个品种‘中花’、‘澄海粗条’和‘全年油青’为材料,对菜薹不同节间(菜薹从膨大处到顶端依次平分为4节)芥子油苷组分和含量进行了鉴定和分析.结果表明,除4-甲硫基丁基芥子油苷在全年油青菜薹的第1节中没有检测到、4-羟基-3-吲哚甲基芥子油苷在全年油青菜薹的第1节位和第2节位中没有检测到外,在3个芥蓝品种菜薹的其它节均检测到11种芥子油苷,包括7种脂肪族芥子油苷(3-甲基亚硫酰丙基芥子油苷、2-羟基-3-丁烯基芥子油苷、2-丙烯基芥子油苷、4-甲基亚硫酰丁基芥子油苷、5-甲基亚硫酰戊基芥子油苷、3-丁烯基芥子油苷和4-甲硫基丁基芥子油苷)和4种吲哚族芥子油苷(4-羟基-3-甲基吲哚芥子油苷、3-吲哚甲基芥子油苷、4-甲氧基-3-吲哚甲基芥子油苷和1-甲氧基-3-吲哚甲基芥子油苷).在3个芥蓝品种中,总芥子油苷、总脂肪族芥子油苷和总吲哚族芥子油苷均以菜薹的第四节含量最高,显著高于其它节,而不同的芥子油苷种类在菜薹各节间的变化趋势不一致.The composition and content of glucosinolates （GSs） in different bolting stem sections （ Bolting stem was divided into four sections; from the bottom to top） of three Chinese kale （Brassica alboglabra L. ） cultivars （CH, CHCT and QNYQ） were analyzed by HPLC and identified by LC-MS. Eleven kinds of GSs were identified including seven aliphatie GSs （glueoiberin, progoitrin, sinigrin, glucoraphanin, glucoalyssin, gluconapin and glucoerucin） and four indolyl GSs （4-hydroxyglucobrassi- cin, glucobrassiein, 4-methoxyglucobrassicin and neoglucobrassincin）, except for that in QNYQ, there was no glucoerucin in the first section and 4-methoxyglucobrassicin in the first and second section of bolting stems identified. In the three Chinese kale cultivars, total GS content, total aliphatic GS content and total indolyl GS content were all achieved in the top section of bolting stems in Chinese kale, however, the change trend of different GS in bolting stem sections were not consistent.

关 键 词：芥蓝 菜薹 芥子油苷 节间 种类 含量 

分 类 号：S635.9[农业科学—蔬菜学] TS201[农业科学—园艺学]