海带多糖对心理应激大鼠血管内皮依赖性舒缩功能的影响  

Effect of Laminaria Polysaccharide on the Endothelium-dependent Relaxation and Contraction of Rats Induced by Psychological Stress

作　　者：黎静[1] 谢露[1] 杨晓梅[1] 庞广保[2] 王圣泳[2] 王界年[2] 沈斌[2] 

机构地区：[1]广西医科大学生理教研室,广西省南宁市530021 [2]广西医科大学

出　　处：《中国动脉硬化杂志》2012年第1期6-10,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目(30960467);国家自然科学青年基金项目(81000148);广西教育厅博士研究生创新课题资助项目(2010105981001D37)

摘　　要：目的研究海带多糖对心理应激大鼠血管内皮依赖性舒张和收缩功能的影响。方法采用空瓶刺激法建立心理应激大鼠模型,大鼠随机分为对照组、模型组、海带多糖低剂量组、海带多糖高剂量组及低分子肝素组。经旷场试验后,取胸主动脉置于恒温灌流系统,分别记录血管环在累积浓度乙酰胆碱、去甲肾上腺素诱导后的血管舒缩反应,以及一氧化氮合酶抑制剂(左旋硝基精氨酸甲酯)孵育后累积浓度硝酸甘油的血管舒张反应情况。结果模型组、海带多糖低剂量组、海带多糖高剂量组和低分子肝素组旷场行为学评分显著高于对照组(P<0.05)。与对照组比较,模型组对乙酰胆碱诱导的血管舒张反应明显降低(P<0.05),对去甲肾上腺素诱导的血管收缩反应明显增强(P<0.05);与模型组比较,海带多糖高剂量组对乙酰胆碱诱导的血管舒张反应明显增强(P<0.05),对去甲肾上腺素引起的血管收缩反应明显减弱(P<0.05),海带多糖低剂量组和低分子肝素组血管舒缩反应无显著性变化(P>0.05)。左旋硝基精氨酸甲酯孵育后,与对照组比较,模型组、海带多糖低剂量组、海带多糖高剂量组和低分子肝素组对硝酸甘油引起的舒张反应均显著降低(P<0.05),海带多糖高剂量组、海带多糖低剂量组和低分子肝素组加入左旋硝基精氨酸甲酯孵育后硝酸甘油诱导的血管最大舒张反应显著低于乙酰胆碱诱导(未加入左旋硝基精氨酸甲酯孵育)的血管最大舒张反应。结论海带多糖对心理应激大鼠血管内皮依赖性舒缩功能具有保护作用。因此,海带多糖可以拮抗内源性肾上腺素增多导致的内皮损伤。Aim To investigate the effect of laminaria polysaccharide （LP） on the vascular endothelium-depend- ent relaxation and contraction of rats induced by psychological stress. Methods Normal adult rats are randomly divid- ed into control group, LP high group （LP-H group）, LP low group （LP-L group） and low molecular heparin group, which was to build psychological stress of rat model by using the stimulation of uncertainty empty bottle. After the stress, rats were reviewed by open-field test. Then aortic rings were isolated and put into organ bath. The tension of endothelium- dependent relaxation induced by acetylcholine （Ach）, contraction induced by norepinephrine （NE） and relaxation induced by nitroglycerin （NTG） after adding in L-arginine methyl ester hydrochloride （L-NAME） were recorded. Results Compared with control group, the frequency of crossing and rearing behavior increased significantly in model group, LP-H group, LP-L group and low molecular heparin groups. The model group reduced Ach-induced relaxation （P 〈 0.05 ） and enhanced NE-induced contraction （P 〈 0.05） in comparison with control group. Compared with model group, the relaxation induced by Ach significantly increased and the contraction induced by NE significantly are decreased in LP-H group. It is meaningless in LP-L group and low molecular heparin group. In model, LP-H group, LP-L group and heparin groups, relaxation induced by nitroglycerin after adding in L-NAME was rather lower than that of control group. The max- imum relaxation induced by nitroglycerin with L-NAME is significantly lower than that of induced by Ach without L-NAME in LP-H group, LP-L group and heparin groups. Conclusions LP can prevent vascular endothelium-dependent relax- ation and contraction of rat induced by psychological stress. So it may prevent endothelium damaged by endogenous adrenaline.

关 键 词：海带多糖 心理应激 内皮依赖性舒张反应 内皮依赖性收缩反应 乙酰胆碱 

分 类 号：R363[医药卫生—病理学]