Characterization of human primary chondrocytes of osteoarthritic cartilage at varying severity  

作　　者：YIN Jing YANG Zheng CAO Yong-ping GE Zi-gang 

机构地区：[1]Department of Biomedical Engineering, College of Engineering,Peking University, Beijing 100871, China [2]Department of Orthopaedic Surgery, Yong Loo Lin School ofMedicine, NUS Tissue Engineering Program, National Universityof Singapore, 119260, Singapore [3]Department of Orthopaedics, Peking University First Hospital, Beijing 100034, China [4]Center for Biomaterials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China

出　　处：《Chinese Medical Journal》2011年第24期4245-4253,共9页中华医学杂志（英文版）

基　　金：This work is supported by National Basic Research Program of China （973 Program） （No. 2011CB707500, 2012CB619102） and National Natural Science Foundation of China （No. 30772198, 30970881, 31011140348）.

摘　　要：Background There is a difficulty in evaluating the in heterogeneity among cartilage affected by osteoarthritis vivo functionality of individual chondrocytes, and there is much （OA）. In this study, in vitro cultured chondrocytes harvested from varying stages of degeneration were studied as a projective model to further understand the pathogenesis of osteoarthritis. Methods Cartilage of varying degeneration of end-stage OA was harvested, while cell yield and matrix glycosaminoglycan （GAG） content were measured. Cell morphology, proliferation, and gene expression of collagen type 1,‘11, and X, aggrecan, matrix metalloproteinase 13 （MMP-13）, and ADAMTS5 of the acquired chondrocytes were measured during subsequent in vitro culture. Results Both the number of cells and the GAG content increased with increasing severity of OA. Cell spreading area increased and gradually showed spindle-like morphology during in vitro culture. Gene expression of collagen type II, collagen type X as well as GAG decreased with severity of cartilage degeneration, while expression of collagen type I increased. Expression of MMP-13 increased with severity of cartilage degeneration, while expression of ADAMTS-5 remained stable. Expression of collagen type II, X, GAG, and MMP-13 substantially decreased with in vitro culture. Expression of collagen type I increased with in vitro cultures, while expression of ADAMTS 5 remained stable. Conclusions Expression of functional genes such as collagen type Ⅱ and GAG decreased during severe degeneration of OA cartilage and in vitro dedifferentiation. Gene expression of collagen I and MMP-13 increased with severity of cartilage degeneration.Background There is a difficulty in evaluating the in heterogeneity among cartilage affected by osteoarthritis vivo functionality of individual chondrocytes, and there is much （OA）. In this study, in vitro cultured chondrocytes harvested from varying stages of degeneration were studied as a projective model to further understand the pathogenesis of osteoarthritis. Methods Cartilage of varying degeneration of end-stage OA was harvested, while cell yield and matrix glycosaminoglycan （GAG） content were measured. Cell morphology, proliferation, and gene expression of collagen type 1,‘11, and X, aggrecan, matrix metalloproteinase 13 （MMP-13）, and ADAMTS5 of the acquired chondrocytes were measured during subsequent in vitro culture. Results Both the number of cells and the GAG content increased with increasing severity of OA. Cell spreading area increased and gradually showed spindle-like morphology during in vitro culture. Gene expression of collagen type II, collagen type X as well as GAG decreased with severity of cartilage degeneration, while expression of collagen type I increased. Expression of MMP-13 increased with severity of cartilage degeneration, while expression of ADAMTS-5 remained stable. Expression of collagen type II, X, GAG, and MMP-13 substantially decreased with in vitro culture. Expression of collagen type I increased with in vitro cultures, while expression of ADAMTS 5 remained stable. Conclusions Expression of functional genes such as collagen type Ⅱ and GAG decreased during severe degeneration of OA cartilage and in vitro dedifferentiation. Gene expression of collagen I and MMP-13 increased with severity of cartilage degeneration.

关 键 词：OSTEOARTHRITIS chondroeyte DIFFERENTIATION 

分 类 号：Q813.11[生物学—生物工程] R87[医药卫生—运动医学]