KiSS-1基因在黄牛精子发生中的表达  

Expression of KiSS-1 gene in bovine testis spermatogenesis

作　　者：邵宝平[1,2] 王继卿[1] 罗玉柱[1] 王建林[2] 陈建龙 柴尔青[2] 徐元青[2] 

机构地区：[1]甘肃省草食动物生物技术重点实验室,甘肃兰州730070 [2]兰州大学生命科学学院,甘肃兰州730000 [3]中农威特生物科技股份有限公司,甘肃兰州730046

出　　处：《中国兽医科学》2011年第12期1282-1287,共6页Chinese Veterinary Science

基　　金：甘肃省重点实验室建设计划项目(085RTSA004);教育部博士点-新教师基金项目(20090211120002);国家自然科学基金青年科学基金项目(31000190)

摘　　要：为探讨亲吻素在黄牛精子发生中的生理功能,并为家畜生殖生理学研究提供参考,运用免疫组织化学SABC染色法并采用Image Pro-Plus 6.0软件,对成年黄牛睾丸中KiSS-1的表达及分布特征进行了研究。结果显示,在成年黄牛睾丸的精原细胞、初级精母细胞、次级精母细胞、早期精子细胞及间质细胞中均发现KiSS-1阳性反应产物,其各阳性细胞积分光密度(IOD)值的大小顺序为IOD初级精母细胞>IOD间质细胞>IOD次级精母细胞>IOD精原细胞>IOD早期精子细胞,而阳性产物在晚期精子细胞、精子和支持细胞及睾丸间质血管上未被发现;在部分间质细胞周围可见大量的KiSS-1免疫阳性反应产物,而在其他阳性细胞周围未见此现象。结果证实,KiSS-1基因在黄牛精子发生中发挥着重要作用,尤其在精原细胞的有丝分裂和精母细胞的减数分裂中可能发挥着非常重要的作用,而且间质细胞具有向睾丸微环境中分泌KiSS-1的功能。To explore the physiologic function of the kisspeptins in bovine spermatogenesis as well as provide reference to research reproductive physiology of livestock,kisspeptins-1（Kiss-1） expression characteristics in the testis of adult cattle were studied by using SABC immunohistochemical staining and image analysis software（Image Pro-Plus6.0）.In result,the KiSS-1 immunopositive production were found in the spermatogonia,primary spermatocytes,secondary spermatocytes,early spermatids and interstitial cells of the adult bovine testes,with the order of the total optical density of the positive cells（IOD） value as IODprimary spermatocytes IODinterstitial cells IODsecondary spermatocytes〉IODspermatogonia 〉IODearly spermatids（P〈0.05）.However,the production were not found in the late spermatids,sperm,sertoli cells and interstitial blood vessels of the adult cattle testes.The large KiSS-1 immunoreactivity production was seen around the periphery of some interstitial cells,while this phenomenon was not found in other positive cells periphery.The results suggested that KiSS-1 gene plays a very important role in the spermatogenesis of cattle testes,and may especially play very important regulative role in the spermatogonia mitosis and spermatocytes meiosis,and the testicular interstitial cells have the function of KiSS-1 secretion in the testes microenvironment.

关 键 词：亲吻素-1 精子发生 睾丸 黄牛 

分 类 号：S852.16[农业科学—基础兽医学]