金莲花化学成分的分离与鉴定(Ⅱ)  

Isolation and identification of chemical constituents from flowers of Trollius chinensis(Ⅱ)

作　　者：魏金霞[1] 李丹毅[1] 华会明[1] 李占林[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2012年第1期12-15,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的研究金莲花(Trollius chinensis Bunge)的化学成分。方法采用硅胶柱色谱及制备薄层色谱等方法进行分离纯化,根据理化性质和波谱数据鉴定化合物结构。结果分离得到14个化合物,分别鉴定为柳穿鱼黄素(pectolinarigenin,1)、刺槐素(acacetin,2)、蓟黄素(cirsimaritin,3)、荭草苷(orientin,4)、2″-O-(2-甲基丁酰基)-异当药黄素(2″-O-(2-methylbutyryl)-isoswertisin,5)、七叶内酯(esculetin,6)、2-(4'-羟基苯基)-1-硝基乙烷(2-(4'-hydroxyphenyl)-1-nitroethane,7)、原儿茶酸(protocatechuic acid,8)、藜芦酸甲酯(methyl veratrate,9)、E-对-香豆酸甲酯(methyl E-p-coumarate,10)、香草酸(vanillic acid,11)、对羟基苯甲酸(p-hydroxybenzonic acid,12)、对羟基苯甲酸甲酯(methyl p-hydroxybenzoate,13)、金莲花碱(trolline,14)。结论化合物1～2,6～10为首次从金莲花属植物中分离得到,化合物11～13为首次从金莲花中分离得到。Objective To isolate and identify the chemical constituents from the flowers of Trollius chinensis. Methods Compounds were isolated by various kinds of column chromatography methods and preparative TLC, and their structures were identified by the physico-chemical characters and the spectral analysis. Resuits Fourteen compounds were isolated and their structures were identified as pectolinarigenin （ 1 ）, acacetin （ 2 ）, cirsimaritin （3）, orientin （ 4 ）, 2＂-0- （ 2＂-methylbutyryl ） -isoswertisin （ 5 ）, esculetin （ 6 ）, 2- （ 4＇-hydroxyphenyl） -1-nitroethane （7）, protocatechuic acid （8）, methyl veratrate （ 9 ）, methyl E-p-coumarate （ 10 ）, vanil- lic acid （ 11 ） ,p-hydroxybenzonic acid （ 12 ）, methyl p-hydroxybenzoate （ 13 ）, and trolline （ 14 ）, respectively. Conclusions Compounds 1-2,6-10 are isolated from Trollius genus for the first time, and compounds 11- 13 are isolated from this plant for the first time .

关 键 词：金莲花 化学成分 结构鉴定 黄酮 有机酸 

分 类 号：R914[医药卫生—药物化学] R284.2[医药卫生—药学]