山楂提取物对脂肪酶活性的抑制作用研究  

Lipase Inhibitory Activity of Extracts of Hawthorn

作　　者：邓克国[1] 李谭瑶[1] 陈波[1] 姚守拙[1] 

机构地区：[1]湖南师范大学化学生物学及中药分析教育部重点实验室,长沙410081

出　　处：《天然产物研究与开发》2011年第B12期164-167,233,共5页Natural Product Research and Development

基　　金：国家自然科学基金（20875028）,国家重点基础研究发展计划（973计划）（2006CB504701）

摘　　要：建立从中药山楂中筛选脂肪酶抑制剂的气相色谱法，探讨山楂提取物对脂肪酶的抑制活性及机理。以三丁酸甘油酯为底物，采用猪胰脂肪酶抑制剂活性筛选模型对各组分进行筛选。通过气相色谱对产物正丁酸与内标物己酸的峰面积比值进行定量分析。在0．10-5．00mmoL／L范围内，正丁酸与内标物己酸的峰面积比值线性关系良好，得到线性方程为Y=1．5269x-0．0723（R2=0．9987）。计算得水、乙醇、氯仿和乙酸乙酯提取物IC50分别为17．44、7．0、2．91me／mL和1．31mg／mL。Dixon图和Lineweaver-Burk图表明山楂提取物对胰脂肪酶的抑制作用为非竞争性抑制，Ki=0．91g／L。实验研究表明，山楂的乙酸乙酯提取物是脂肪酶的有效抑制剂。A gas chromatography method was devdoped for screening lipase inhibitors from hawthorn,and discussed the inhibitory activity and mechanism of the extract of hawthorn on lipase. Using activity model of porcine pancreatic lipase inhibitor for screening activity component with tributyrin as substrate, and quantitative analysis of the ratio of peak area of butyric acid hydrelyzed to internal standard caproic acid by gas chromatography. In the range of 0. 10-5.00 retool/L, the ratio of butyric acid hydrolyzed to caproic acid was in good linear relationship. The calculated IC50 values of extraction part of water, ethanol, chloroform and acetyl acetate were 17.44,7.00,2.91 mg/mL, and 1.31 mg/mL, respectively. The inhibitory mode of the extract of hawthorn, which was determined by the Lineweaver-Burk plot analysis, was a non-competitive inhibitor. The inhibitory content Ki value was 0.91 mg/L. Experimental results show that the ethyl acetate extract of hawthorn is an effective inhibitor of lipasc.

关 键 词：脂肪酶 山楂提取物 抑制活性 非竞争性抑制 

分 类 号：Q946.91[生物学—植物学] R285[医药卫生—中药学]