牛乳腺炎金黄葡萄球菌肠毒素B基因的克隆及序列分析  

Cloning and sequencing of SEB gene of Staphylococcus aureus from mastitis cows

作　　者：薛晓阳[1] 布日额[2] 吴金花[2] 刘洋[1] 

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028043 [2]内蒙古民族大学生命科学学院,内蒙古通辽028043

出　　处：《中国病原生物学杂志》2011年第12期891-893,共3页Journal of Pathogen Biology

基　　金：国家自然科学基金项目(No.31060325);内蒙古自治区科技厅科技创新引导计划项目(2011年)

摘　　要：目的克隆牛乳腺炎金黄葡萄球菌B(SEB)基因,并进行序列分析。方法根据GenBank公布的金黄葡萄球菌基因的全序列,利用生物学软件Primer5.0和Oligo 6.0设计1对特异性引物,扩增临床分离菌株的SEB基因片段。结果扩增的基因片序列长度为466bp,与标准菌株(CMCC26074)的SEB基因片段序列相似性为100%,与GenBank公布的金黄葡萄球菌菌株(AB479118.1)SEB基因序列相似性为99.93%。结论成功克隆出牛乳腺炎金黄葡萄球菌SEB基因,为建立相应的PCR快速检测牛乳SEB基因方法奠定了基础。Objective Cloning and sequencing of bovine mastitis Staphylococcus aureus SEB gene. Methods A pair of primers were designed by biological software of primer 5.0 and Oligo 6.0 according to SEB gene sequences of bovine mastitis S.aureus published on GenBank.SEB gene fragment of clinical isolated strain was amplified by PCR. ResultsSequencing result showed that SEB gene sequences had 466 bp in length.SEB gene sequences of clinical isolated strain had 100% of homology with the standard strain（CMCC26074） in gene sequences,and had 99.93% of homology with the SEB gene sequences of standard S.aureus strain（AB479118.1） published on GenBank.Conclusion The bovine mastitis S.aureus SEB gene had been cloned.This result had laied a foundation for further established of a rapid detection method for SEB gene in dairy milk.

关 键 词：金黄葡萄球菌 肠毒素B基因 克隆 序列分析 

分 类 号：R378.11[医药卫生—病原生物学] S858.23[医药卫生—基础医学]