微波辅助固相微萃取法分析光皮桦叶片和果实中挥发性成分  

Analysis of Volatile Composition of Leaf and Fruit in Betula luminifera by MAE-HS-SPME

作　　者：杨再波[1] 龙成梅[1] 郭治友[2] 毛海立[1] 孙成斌[1] 

机构地区：[1]黔南民族师范学院化学与化工系,贵州都匀558000 [2]黔南民族师范学院生命科学系,贵州都匀558000

出　　处：《中国实验方剂学杂志》2012年第1期56-59,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省教育厅2010年度自然科学研究基金项目(黔教科2010094);贵州省黔南民族师范学院2009年度重点资助科研项目(QNSY0915)

摘　　要：目的:应用气相色谱-质谱法(GC-MS)分析光皮桦叶片和果实中的挥发性成分。方法:采用微波辅助顶空固相微萃取技术分别提取光皮桦叶片和果实中的主要挥发性成分,用气相色谱-质谱法与色谱保留指数相结合进行定性定量分析。结果:从光皮桦叶片和果实中分别鉴定出58,25个化合物,分别占挥发性组分总峰面积的95.78%,95.72%,在叶片中主要成分是十五烷(5.50%),十六烷(6.54%)和(E)-1,2,3-三甲基-4-丙烯基-萘(5.13%);而果实中主要成分是顺式-β-金合欢烯(10.04%),β-甜没药烯(24.81%),α-石竹烯(25.61%)和α-甜没药醇(16.42%)。结论:两个部位的挥发性成分存在较大差异。Objective：To analyze and compare the volatile constituents which were extracted from the different parts of Betula luminifera by GC-MS.Method：The volatile components were extracted from the different parts of B.luminifera by t MAE-HS-SPME and the chemical components of them were identified by GC-MS and retention index.Result：58 and 25 volatile constituents in the leaf and fruits of Betula luminifera were separated and identified.Accounting for 95.78% and 95.72% of the total peak areas,respectively.The major components in the leaf oil were pentadecane（5.50%）,hexadecane（6.54%）,（E）-1,2,3-trimethyl-4-propenyl-naphthaleene（5.13%）,whereas the major constituents in the fruit oil were cis-β-farnesene（10.04%）,β-bisabolene（24.81%）,α-caryophyllene（25.61%） and α-bisabolol（16.42%）.Conclusion：The results show that the two parts of the volatile components are quite different.

关 键 词：光皮桦 叶片和果实 挥发性成分 微波辅助顶空固相微萃取 气相色谱-质谱 

分 类 号：R284.1[医药卫生—中药学]