反义寡核苷酸联合磁性载药微球靶向治疗耐药裸鼠人胰腺癌  

Reversal of multidrug resistance antisense oligodeoxynucletedes and 5-fluorouracil magnetic albu-min miscrospheres on human pancreatic carcinoma model in nude mice

作　　者：潘耀振[1] 孙诚谊[1] 权广前[1] 喻超[1] 

机构地区：[1]贵阳医学院附属医院肝胆外科,550004

出　　处：《中华实验外科杂志》2012年第1期23-25,共3页Chinese Journal of Experimental Surgery

基　　金：贵州省省长基金资助项目[黔省专合字（2006）62号]

摘　　要：目的观察辐射及多药耐药基因逆转剂反义寡核苷酸（mdr1ASON）联合5一氟尿嘧啶磁性白蛋白微球（5一Fu-MAMS）靶向治疗耐药裸鼠人胰腺癌模型的效果。方法构建人胰腺癌耐药细胞株（SW1990／Fu），建立裸鼠人胰腺癌耐药模型，选择适当的磁场，施加于肿瘤表面，瘤内注射mdr1ASON及5一Fu．MAMS，观察肿瘤生长，检测其对耐药裸鼠人胰腺癌的治疗效果。结果在外加磁场的磁导向作用下，5-Fu-MAMS能定位于肿瘤组织，联合mdr1ASON能显著抑制肿瘤生长，肿瘤体积抑制率达到85．00％，肿瘤重量抑制率达到87．74％，与其他组比较差异有统计学意义（P〈0．01）。结论辐射促转染的mdr1ASON联合磁性载药微球对肿瘤细胞具有较好的耐药逆转作用。Objective To investigate the reversal the muhidrug resisitanee （MDR） by MDR gene mdrl antisense oligodeoxynueletdes （ASON） and 5-fluorouraeil magnetie albumin miserospheres （5-Fu- MAMS） magnetic targeted therapy the human pancreatic cancer in nude mouse model. Methods To construct SW1990/Fu and nude mice experimental model, to investigate the targeted therapy effects of mdrlASON and 5-Fu-MAMS. Results 5-Fu-MAMS magnetic response was good, its could be well localized in the tumor tissue and markedly inhibit the growth of tumor. The inhibitory rate of tumor size was 85.00%, and that of tumor weight 87.74%. which can effectively degrade proliferating index and accelerate cell death of tumor cell. There were significant differences as compared with other groups（P 〈 0. 01 ）. Conclusion Mdrl ASON and 5-Fu-MAMS had outstanding reverse effect drug fast on human pancreatic carcinoma model in nude mice.

关 键 词：反义寡核苷酸 5-氟尿嘧啶 磁性白蛋白微球 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]