PD-L1mRNA与PD-L2mRNA在口腔扁平苔藓中的表达特点及可能作用  

Study on the expression and potential effect of PD-L1mRNA and PD-L2mRNA in oral lichen planus

作　　者：杜观环[1] 秦孝鹏[1] 李琴[1] 周永梅[1] 沈雪敏[1] 唐国瑶[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔黏膜病科,上海市口腔医学重点实验室,上海200011

出　　处：《上海口腔医学》2011年第6期619-622,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金(30872888);上海市科学技术委员会资助项目(08DZ2271100);上海市重点学科建设项目(S30206)

摘　　要：目的:研究PD-L1mRNA、PD-L2mRNA在口腔扁平苔藓(oral lichen planus,OLP)患者外周血与损害组织,以及在不同临床类型与病理分型中的表达特点。方法:采用实时定量PCR方法检测60例OLP患者(斑纹型35例,充血糜烂型25例;不伴异常增生38例,伴轻度异常增生22例)PD-L1mRNA、PD-L2mRNA表达水平,并以10名正常人外周血与黏膜组织作为对照,比较不同临床类型和病理类型OLP患者间PD-L1mRNA、PD-L2mRNA表达水平差异,并分析PD-L1mRNA、PD-L2mRNA在OLP患者外周血与损害组织中表达的相关性。利用SAS6.12软件包中的非配对两样本均数比较的Wilcoxon秩和检验,比较不同组别间的差异。结果:PD-L2mRNA在OLP患者外周血中表达降低,损害组织中表达升高,且在不同临床类型OLP患者之间存在显著差异(P<0.05)。PD-L1mRNA在OLP组与正常对照组之间的表达差异无统计学意义(P>0.05)。结论:PD-L2分子可能在OLP患者全身与损害局部对OLP的发生、发展产生不同的影响。PURPOSE：To study the expression of PD-L1 and PD-L2mRNA in oral lichen planus（OLP） patients＇ peripheral blood and focal mucosa,and the different expression of target molecules in gene level in different clinical and pathological OLP groups.METHODS： The expression of PD-L1 and PD-L2 mRNA in OLP patients,which included 35 reticular and 25 atrophic-erosive OLP patients,38 cases without dysplasia and 22 cases with dysplasia,was examined by real-time PCR.Peripheral blood and mucosa from 10 volunteers were used as control.All the results were analysed with Wilcoxon test by SAS.6.12 software package.RESULTS： The expression of PD-L2mRAN,but not PD-L1,was significantly higher in oral mucosa of OLP patients（P0.01）,while decreased in OLP patients＇ peripheral blood（P=0.0415）.The gene expression of PD-L2 differed between different clinical types,and had highly significant correlation between the OLP patients＇ focal mucosa and peripheral blood（r=0.6976,P0.0001）.CONCLUSIONS： PD-L2 may have some potential effect on the pathogenesis of OLP at systemic and local level.Supported by National Natural Science Foundation of China（30872888）,Research Fund of Science and Technology Commission of Shanghai Municipality（08DZ2271100） and Shanghai Leading Academic Discipline Project（S30206）.

关 键 词：口腔扁平苔藓 PD-L2 PD-L1 定时定点PCR 

分 类 号：R781.5[医药卫生—口腔医学]