施用茶树菇栽培废料对青菜土壤中微生物学特征的影响  

Influence of Agrocybe Aegerita Residue on the Characteristics of Soil Microbe Under Green Vegetables Cultivation

作　　者：徐江兵[1,2,3] 林先贵[1,2] 王一明[1,2] 王俊华[1,2] 

机构地区：[1]中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京210008 [2]中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,南京210008 [3]中国科学院研究生院,北京100049

出　　处：《农业环境科学学报》2012年第1期131-136,共6页Journal of Agro-Environment Science

基　　金：中国科学院知识创新工程重大项目(KSCX2-EW-N-08);948项目(2011-G25)

摘　　要：通过温室盆栽实验研究了施用茶树菇栽培废料对青菜生物量及株高、土壤微生物数量、酶活性及碳源利用能力的影响。结果表明,施用茶树菇栽培废料显著增加了青菜生物量及土壤中放线菌、有机解磷菌、无机解磷菌、纤维素降解菌的数量,改善了土壤微生物区系,提高了土壤磷酸酶活性。单碳源利用图谱显示,施用茶树菇栽培废料增加了土壤微生物功能多样性指数(Shannon指数),降低了均一性指数(McIntosh指数)。主成分分析结果表明,施用茶树菇栽培废料导致土壤微生物主要对糖类物质的利用能力产生分异。研究表明茶树菇栽培废料可作为一种良好的有机肥料,通过增加非优势土壤微生物的功能多样性而改善土壤微生物特性,从而加速土壤碳、磷等元素循环以促进作物生长和增加产量。A pot experiment was carried out to investigate the influence of Agrocybe Aegerita residue on biomass and height of green vegetables,and soil microbial numbers,enzyme activities,ustilization ability of carbon sources.The numbers of actinomycetes,phosphate-degradating bacteria,cellulose-degradating bacteria and the microbiota was improved as Agrocybe Aegerita residue input increased.Results of BIOLOG showed that the more input of Agrocybe Aegerita residue,the larger the functional diversity index（Shannon-Wiener Index） but the smaller the evenness index（McIntosh Index）.PCA analysis showed there were different carbon substrate utilization patterns among treatments,and carbohydrates were main cause of variations.Our results suggest that the Agrocybe Aegerita residue could be used as favourable organic fertilizer,which could improve the characteristics of soil microbes through increasing microbial functional diversity,then accelerating the cycle of carbon and phosphate,thus promoting plants growth and crop yield.

关 键 词：茶树菇 栽培废料 土壤酶 BIOLOG 功能多样性 

分 类 号：S181[农业科学—农业基础科学]