罗氏乳杆菌无细胞上清培养液移除胆固醇能力的研究  

Cholesterol-Reducing Activity in a Cell-Free Supernatant of Lactobacillus reuteri

作　　者：于瑞莉[1] 郭本恒[1,2] 张灏[1] 吴正钧[2] 王荫榆[2] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,无锡214122 [2]光明乳业股份有限公司技术中心乳业生物技术国家重点实验室,上海200436

出　　处：《天然产物研究与开发》2012年第1期89-93,共5页Natural Product Research and Development

基　　金：上海市科技委员会乳业生物工程技术研究中心(09DZ2251400);上海市科技委员会乳业生物技术国家重点实验室筹建(10dz2221100)

摘　　要：本文探讨了罗氏乳杆菌DSM122460无细胞上清培养液(Cell-Free Supernatant,CFS)移除胆固醇的能力。采用邻苯二甲醛法测定DSM122460和对照菌株ST-III发酵过程中及其CFS对胆固醇的移除能力,并研究不同CFS浓度下的移除能力。并采用HPLC法测定CFS对照、热处理组和pH7.0组的胆盐水解酶活力,同时测定其移除胆固醇能力。结果显示,DSM122460不仅在发酵过程中具有较高的移除胆固醇能力,其CFS也表现出较高的移除能力,CFS中含有除胆盐水解酶以外的可移除胆固醇的蛋白类成分。这提示可能存在一种乳酸菌移除胆固醇的新机制。The cholesterol removal ability of cell-free supernatant（CFS） produced by growth of Lactobacillus reuteri DSM122460 in MRS broth was investigated.The cholesterol removal ability of L.reuteri train DSM122460 as well as L.plantarum strain ST-III during growth in MRS and the resulted CFS was assayed by o-phthalaldehyde method,respectively.In order to elucite the correlation between BSH activity and the cholesterol removal ability of the Lactobacillus strains,BSH roles in cholesterol removal were measured by heat treatment and pH adjustment.The remaining BSH activities after various treatments were measured by HPLC.The results showed that L.reuteri strain DSM122460 not only had a high ability to remove cholesterol during its growth in MRS broth,it aslo demonstrated high cholesterol reduction activity in the CFS.Beside the BSH,it was speculated there may be another effective component in the CFS with the ability to remove cholesterol.It suggested that there might be a new mechanism underlying cholesterol reduction by lactic acid bacteria.

关 键 词：罗氏乳杆菌 CFS 移除胆固醇 BSH HPLC 

分 类 号：TS201.3[轻工技术与工程—食品科学]