神经母细胞瘤恶性进展相关基因初步筛选及验证  

Screening and Identification of Candidate Genes Associated with Progression of Neuroblastoma

作　　者：于萌[1] 朱育焱[2] 杨春明[2] 王海波[2] 李元元 中川原章 郑志红[1] 

机构地区：[1]中国医科大学实验动物部转基因实验室,沈阳110001 [2]中国医科大学附属第一医院泌尿外科,沈阳110001 [3]日本千叶癌中心研究局肿瘤基因组学研究室,日本千叶2608718

出　　处：《中国医科大学学报》2012年第2期148-150,157,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(31000572);教育部新教师基金资助项目(20102104120013);辽宁省博士科研启动基金资助项目(20101148);辽宁省教育厅高校科研计划(L2010622)

摘　　要：目的利用神经母细胞瘤(NB)临床标本筛选与恶性进展相关的基因。方法利用比较基因组杂交技术和表达谱基因芯片技术筛选人类17号染色体上某些区域可能存在的与NB密切相关的候选基因,并结合生物信息学分析和RT-PCR检测结果对候选基因进行进一步鉴定。结果通过初步筛选,在人类17号染色体增添区域发现了与NB恶性进展相关的新基因(ncRAN),该基因包含2个剪切体,Nbla10727和Nbla12061。ncRAN在各组织中及MYCN单/多拷贝组细胞中呈现差异性表达,其高表达与不良预后密切相关(P=0.000 221)。结论在17号染色体增添区所鉴定的新基因很可能是1个与NB恶性进展密切相关的癌基因。Objective To discover the molecular mechanisms underlying progression of human neuroblastoma by screening the candidate genes in clinical specimens.Methods The candidate genes mapped on human chromosome 17q gain,which was significantly associated with the neuroblastoma,were screened by combining the array-CGH results with expression profiling,and identified by bioinformatic analysis and RT-PCR.Results A novel gene linked to progression of neuroblastoma named ncRAN was found on chromosome 17q gain,which included two transcripts,Nbla10727 and Nbla12061,and differential expression can be detected in various tissues and neuroblastoma cell lines with a single copy and amplification of MYCN.Furthermore,high expression of ncRAN was associated with poor prognosis（P =0.000 221）.Conclusion The novel gene mapped on chromosome 17q gain may be an oncogene which is associated with progression of neuroblastoma.

关 键 词：神经母细胞瘤 17号染色体 癌基因 CDNA芯片 

分 类 号：R73[医药卫生—肿瘤]