几株赤潮甲藻的摄食能力  

Ingestion of selected HAB-forming dinoflagellates

作　　者：张清春[1,2] 于仁成[1] 宋静静[1,2] 颜天[1] 王云峰[1] 周名江[1] 

机构地区：[1]中国科学院海洋研究所,海洋生态与环境科学重点实验室,青岛266071 [2]中国科学院研究生院,北京100049

出　　处：《生态学报》2012年第2期402-413,共12页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(40606029);国家重点基础研究发展计划项目(973项目)(2010CB428705);国家基金委创新研究群体科学基金项目(40821004)

摘　　要：采用荧光标记的方法,在营养盐限制条件下,对6株赤潮甲藻对荧光标记的海洋细菌(FLB)、金藻(FLA)和两种粒径分别为0.5μm和2.0μm的荧光微球(FM0.5和FM2.0)4种摄食对象的摄食进行了比较研究。研究结果表明,除了东海原甲藻对4种摄食对象均没有摄食外,其它5株甲藻,微小亚历山大藻、链状亚历山大藻、塔玛亚历山大藻、海洋原甲藻和微小原甲藻均具有摄食能力,但对摄食对象的选择和摄食率有差异,多数摄食率是在4 h达到最大,白天的摄食能力强于夜间。研究说明了在营养盐限制环境中,有些具有兼性营养能力的甲藻对细菌和/或更小浮游植物的摄食能力可能对维持和促进其生长具有不可忽视的作用。The ingestion behavior of six dinoflagellate species isolated from the Chinese and Korean coasts was evaluated in this paper. These six species include Prorocentrum donghaiense, P. micans, P. minimum, Alexandrium minutum, A. catenella, and A. tamarense, which often contribute to the formation of harmful algal blooms （HABs）. They were cultured under either 45 or 8 μEm^-2 s^-1light intensities, in nutrient-depleted culture medium containing different particles, such as fluorescently labeled dead marine bacteria （FLB） , the dead marine micro-flagellate Isochrysis galbana （FLA） , and the fluorescent microspheres of two sizes （ FM0. 5 and FM2. 0, sphere diameter 0.5 and 2.0 μm） , respectively. The ingestion activities were quantified by calculating the percentage of dinoflagellates that ingested either FLB, FLA, FM0. 5, or FM2. 0, based on the observation of 100 dinoflagellate cells in each group. In our experiment, the ingestion behaviors were observed in A. minutum, A. catenella, A. tamarense, P. micans, and P. minimum. And they all showed great different efficiency in choosing different particle to ingest. For example, A. minutum could ingest both FM0.5 and FLA, though a higher percentage of individuals exhibited ingestion behavior under 45 μEm^-2 s^-1 light intensity （ 15 % ） than under 8 μEm^-2 S^-1（2% ）. Similarly, A. catenella ingested FLB, FM0.5, and FLA （5.5%, 7.5%, and 6% individuals of the total population to ingest, respectively） under both light intensities. A. tamarense was able to ingest only FLB and FM0.5 under the low light intensity （7% and 9% of individuals, respectively）. P. micans i 15.5% , and 4. 5% of individuals, respectively） under both light intensities. ngested FLB, FM0.5, and FLA （ 12.5%, A low percentage （9%） of P. minimumindividuals ingested FM0.5, and we observed ingestion under both light intensities. At the same time, it is found that the ingestion behavior showed a peak at 4 h after the ingestion objects were introduced to the medium, and i

关 键 词：东海原甲藻 微小亚历山大藻 链状亚历山大藻 塔玛亚历山大藻 海洋原甲藻 微小原甲藻 海洋细菌 金藻 荧光微球 摄食 

分 类 号：X55[环境科学与工程—环境工程] X173