埃兹蛋白、上皮型钙黏着蛋白、桩蛋白、整合素β1蛋白在皮肤鳞状细胞癌中的表达  

Expressions of ezrin, E-cadherin, paxillin and integrin β1 in skin squamous cell carcinoma

作　　者：闫越颖[1] 张士发[1] 赵丽萍[1] 刘静[1] 刘超[1] 李淑琴[1] 

机构地区：[1]沈阳军区总医院皮肤科,110016

出　　处：《中华皮肤科杂志》2012年第1期49-52,共4页Chinese Journal of Dermatology

摘　　要：目的 探讨埃兹蛋白（ezrin）、上皮型钙黏着蛋白（E-cadherin）、桩蛋白（paxillin）和整合素β1（INTβ1）在皮肤鳞状细胞癌（SCC）中的表达及意义.方法 应用免疫组化法对30例SCC患者及10例正常对照者的ezrin、E-cadherin、paxillin和INTβ1表达进行检测.结果 正常人表皮角质形成细胞上均表达ezrin和E-cadherin（10/10）,表达率显著高于SCC肿瘤细胞[56.67％（17/30）和13.33％（4/30）,x2分别为6.42和24.76,P值均＜0.05]；正常人表皮角质形成细胞上paxillin和INTβ1的表达率为0和10.00％（1/10）,均显著低于SCC肿瘤细胞[70.00％（21/30）和66.67％（20/30）,x2分别为14.74和9.66,P值分别＜ 0.01和0.05].SCC肿瘤细胞ezrin和E-cadherin的表达呈显著正相关（r=0.52,P＜ 0.01）,paxillin和E-cadherin、INTβ1与E-cadherin的表达均呈显著负相关（r值分别为-0.56和-0.52,P值均＜0.01）；ezrin与paxillin、Ezrin与INTβ1、paxillin与INTβ1的表达无相关性（r值分别为0.5、0.24和0,P值均＞0.05）.结论 E-cadherin低表达、paxillin和INTβ1高表达可能共同促进肿瘤细胞的侵袭和转移；INTβ1与E-cadherin在SCC转移中可能起到拮抗作用.Objective To investigate the expressions of ezrin,E-cadherin,paxillin and integrin β1 in skin squamous cell carcinoma （SCC） and their significance.Methods The expressions of ezrin,E-cadherin,paxillin and.integrin β1 were immunohistochemically detected in tissue specimens from 30 patients with SCC and 10 normal human controls.Results The expression rates of ezrin and E-cadherin in normal human keratinocytes were significantly higher than those in SCC cells [ 100.00% （10/10）vs.56.67%（17/30）,x2 =6.42,P 〈 0.05; 100.00% （10/10） vs 13.33% （4/30）,x2=24.76,P 〈 0.05],while the expression rates of paxillin and integrin β1 in normal human keratinocytes were significantly lower than those in SCC cells [0.00vs.70.00% （21/30）,x2 =14.74,P 〈 0.01; 10.00% （1/10） vs.66.67% （20/30）,x2 =9.66,P 〈 0.05].In SCC cells,there was a significant positive correlation between the expression of ezrin and E-cadherin （r =0.52,P 〈0.01 ）,but a negative correlation between the expression of paxillin and E-cadherin and between that of integrin β1 and E-cadherin （r =- 0.56,- 0.52 respectively,both P 〈 0.01）; no significant correlation was observed between the expression of ezrin and paxillin or integrin β1,or between the that of paxillin and integrin β1 （r =0.5,0.24 and 0 respectively,all P 〉 0.05）.Conclusions The low expression of E-cadherin and high expression of paxillin and integrin β1 may synergistically promote the invasion and metastasis of tumor cells,while integrin β1 and E-cadherin may play an antagonistic role in the metastasis of SCC.

关 键 词：皮肤鳞状细胞癌 埃兹蛋白 E-cadherin 上皮型 桩蛋白 整合素 PAXILLIN β1蛋白 

分 类 号：R739.5[医药卫生—肿瘤]