Cyclin D1,Cyclin E,c-Myc在涎腺良恶性多形性腺瘤中的表达研究  

Expression of Cyclin D1,Cyclin E,C-myc in Benign and Malignant Pleomorphic Adenoma of Salivary Gland

作　　者：冯晓洁[1] 罗欣[1] 陈洪伟[1] 温黎明[1] 程勇[2] 

机构地区：[1]河北联合大学口腔医学院口腔颌面外科,河北唐山063000 [2]武汉大学口腔医学院医务处,河北唐山063000

出　　处：《口腔医学研究》2011年第12期1089-1093,共5页Journal of Oral Science Research

摘　　要：目的:研究cyclin D1,cyclin E,c-myc在涎腺多形性腺瘤中的表达,与临床生物学特性和细胞增殖的关系以及对于涎腺多形性腺瘤病理学诊断的意义。方法:采用免疫组化方法检测30例良性多形性腺瘤,30例细胞丰富型多形性腺瘤和30例恶性多形性腺瘤中cyclin D1、cyclin E、c-myc蛋白的表达水平,并与30例癌旁正常涎腺组织中的表达对比。结果:cyclin D1、cyclin E和c-myc在恶性多形性腺瘤中的阳性表达率明显高于良性和细胞丰富型多形性腺瘤(P<0.05),而三者在良性多形性腺瘤中的阳性表达率与在细胞丰富型多形性腺瘤的阳性表达率无统计学差异。cyclin E、cyclin D1和c-myc蛋白的异常表达与患者性别、是否复发、发生部位、肿瘤的大小无关(P<0.05)。在恶性肿瘤中,cyclin D1的异常表达与肿瘤的TNM分期相关(P<0.05),cyclin E蛋白的表达于肿瘤的浸润性相关(P<0.05)。c-myc的过表达与cyclin D1和cyclin E的阳性表达呈正相关。结论:cyclin D1、cyclinE、c-myc共同参与调控细胞周期,可作为预测多形性腺瘤恶变和预后的分子生物学指标之一,是多形性腺瘤病理学分类的依据之一。Objective： To study the expressions of cyclin D1,cyclin E and c-myc in benign and malignant PA,and to learn their association with clinical features and cell proliferation.Methods： SP immunohistochemical staining was applied to detect the expression levels of cyclin D1,cyclin E and c-myc in 30 benign pleomorphic adenomas,30 cellular-type pleomorphic adenomas,30 malignant pleomorphic adenomas,and compared their expression with 30 normal gland tissues of adjacent carcinoma.Results： Cyclin D1,cyclin E and c-myc expression were significantly higher in PA than in the normal gland tissues of adjacent carcinoma（P0.05）.And there was no statistic difference for the expression levels of cyclin D1,cyclin E,c-myc between benign pleomorphic adenoma and cellular-type pleomorphic adenoma.The expression levels of cyclin D1,cyclin E and c-myc were uncorrelated with sex,recurrence,location,and tumor size.But in malignant pleomorphic adenoma,the expression level of cyclin D1 was correlated with TNM stage and the expression level of cyclin E was correlated with invasion.Conclusion： Together or alone of cyclin D1,cyclin E and c-myc might be useful molecular biological markers in predicting the formation,development and carcinogenesis of PA.They might be thought as prognostic markers and chemotherapy targets.They might also contribute to classification of pathology diagnosis.

关 键 词：多形性腺瘤 恶性多形性腺瘤 CYCLIN D1 CYCLIN E C-MYC 免疫组化 

分 类 号：R739.87[医药卫生—肿瘤]