恒化培养稀释率和碳氮比对圆红冬孢酵母油脂积累的影响  

Effects of dilution rate and carbon-to-nitrogen ratio on lipid accumulation by Rhodosporidium toruloides under chemostat conditions

作　　者：沈宏伟[1,2] 靳国杰[2] 胡翠敏[2] 龚志伟[2] 白凤武[1,2] 赵宗保[2,3] 

机构地区：[1]大连理工大学生命科学与技术学院,辽宁大连116023 [2]中国科学院大连化学物理研究所,辽宁大连116023 [3]大连洁净能源剧家实验室(筹),辽宁大连116023

出　　处：《生物工程学报》2012年第1期56-64,共9页Chinese Journal of Biotechnology

基　　金：中国科学院大连化学物理研究所青年科学基金(No.s201027)资助~~

摘　　要：采用恒化培养的方法,考察了稀释率(D)和碳氮比(mol/mol)对圆红冬孢酵母Rhodosporidiumtoruloides AS 2.138 9积累油脂的影响。结果表明:稀释率增大,油脂含量和油脂得率降低。在D=0.02 h 1时油脂得率最大,为0.18 g油/g糖;D=0.14 h 1时油脂生成速率最大,为0.09 g/(L.h)。碳氮比增大,油脂含量略有增加。在C/N=92时油脂得率最大,为0.12 g油/g糖;C/N=32时油脂生成速率最大,为0.13 g/(L.h)。碳氮比对油脂的脂肪酸组成影响不明显,油脂的棕榈酸、硬脂酸和油酸总含量超过85%。The objective of this work is to investigate how dilution rate and carbon-to-nitrogen（C/N） ratio affects lipid accumulation by Rhodosporidium toruloides AS 2.138 9 in continuous culture.Under steady-state conditions,the increase in dilution rate led to the decrease in lipid content and lipid yield.The highest lipid yield and lipid content at D=0.02 h？1 were 0.18 g lipid/g sugar and 57.1%,respectively,while the highest lipid productivity and biomass productivity were obtained at D=0.14 h？1.The increase in C/N ratio led to the increase in lipid content.The highest lipid content of 38% was obtained at C/N=237.The highest lipid yield of 0.12 g lipid/g sugar was obtained at C/N=92.However,the highest lipid productivity of 0.12 g/（L？h） was obtained at C/N=32.No significant changes were observed in terms of fatty acid composition of the lipid produced under different C/N ratios,and these three fatty acids,palmitic acid,stearic acid and oleic acid,took over 85% in all samples.

关 键 词：圆红冬孢酵母 恒化培养 稀释率 碳氮比 油脂 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]