白介素-1β在缓激肽开放血脑屏障过程中的作用及其机制  

Effect and mechanism of interleukin-1β in process of opening the blood-brain barrier by bradykinin

作　　者：秦丽娟[1,2] 薛一雪[1] 谷艳婷[3] 张志勇[4] 张田[2] 孙娜[2] 王东春[5] 宋鸿艳 

机构地区：[1]中国医科大学基础医学院神经生物学教研室,辽宁沈阳110001 [2]河北联合大学基础医学院生理学教研室,河北唐山063000 [3]沈阳药科大学生命科学与生物制药学院生理学教研室,辽宁沈阳110016 [4]河北联合大学附属医院神经外科 [5]河北联合大学附属唐山工人医院神经外科,河北唐山063000 [6]赤峰市松山医院内科,内蒙古赤峰024005

出　　处：《中国药理学通报》2012年第1期58-61,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81101912);国家教育部新教师基金(No 20102134120007);河北省卫生厅科研基金项目(No 20100464;20110165);河北联合大学博士启动基金项目(No BS09012);河北联合大学大学生创新性实验计划项目(No X2011035);辽宁省博士启动基金(No20101109)

摘　　要：目的探讨白介素-1β(IL-1β)在缓激肽(BK)开放血脑屏障(BBB)过程中的作用及其机制。方法缓激肽处理C6细胞后,动态观察培养液中IL-1β含量(放射免疫法)、C6细胞内热休克因子1(HSF1)蛋白的表达(Western blot法)及IL-1β的mRNA水平(RT-PCR法)。利用伊文思蓝检测C6恶性胶质瘤大鼠经颈内动脉给予IL-1β及缓激肽后血脑屏障的通透性。结果缓激肽作用于C6细胞后,培养液中IL-1β的含量明显增加,于120 min含量最多,其后开始减少。C6细胞内HSF1的表达及IL-1β的mRNA水平也在给予缓激肽后明显增加,并分别于干预后的30 min和60 min达高峰后逐渐减少。缓激肽与IL-1β单独作用于C6动物后均可引起胶质瘤大鼠的血脑屏障通透性增加,且IL-1β对肿瘤模型动物血脑屏障通透性的影响与C6细胞培养液中IL-1β的含量相一致。结论 IL-1β可能介导了缓激肽开放血脑屏障的作用,此作用可能是由于缓激肽诱导C6细胞内HSF1的表达增加,增加的HSF1促进神经胶质瘤细胞释放IL-1β所致。Aim To investigate the effects of interleukin-1β（IL-1β） in process of opening blood-brain barrier（BBB） by bradykinin（BK） and its mechanism.Methods Contents of IL-1β in nutrient fluid（radioimmunity method）,HSF1 protein expression（by Western blot） and levels of IL-1β the mRNA（by RT-PCR method） for C6 cells were dynamically observed,after BK treatment.Using Evans blue was applied to detect permeability of BBB after intracarotid infusion of IL-1β for C6 rats.Results For C6 cells,contents of IL-1β in the nutrient fluid were obviously increased after BK treatment and achieved the peak at 120 min,also BK significantly increased expressions of HSF1 and levels of IL-1β mRNA in C6 cells and reached the peak at 30 min and 60 min,separately.Permeability of BBB of C6 animals was in conformity with concentrations of IL-1β in nutrient fluid after BK treatment.Conclusion The IL-1β may mediate the effects of opening the BBB by bradykinin,which may be related with increased HSF1 expression caused by BK and the increased HSF1 promoted neurospongioma cell to release IL-1β.

关 键 词：白介素-1Β 热休克因子1 缓激肽 血脑屏障 血肿瘤屏障 胶质瘤 

分 类 号：R-332[医药卫生] R322.81