基于光滑球拟酵母环境适应性的丙酮酸中试条件优化  

Optimization of Pyruvate Production Conditions Based on Environmental Adaptability of Torulopsis glabrata in a Pilot Plant Scale

作　　者：杨松鑫[1] 王蒙[1] 汪军[1] 刘立明[1] 陈坚[1] 

机构地区：[1]江南大学食品科学与技术国家重点实验室,工业生物技术教育部重点实验室,糖化学与生物技术教育部重点实验室,江苏无锡214122

出　　处：《过程工程学报》2011年第6期1044-1049,共6页The Chinese Journal of Process Engineering

基　　金：全国优秀博士学位论文作者专项基金资助项目(编号:200962);教育部新世纪优秀人才基金资助项目(编号:NCET-10-0456)

摘　　要：在对比研究3和30L规模发酵罐中T.glabrata生产丙酮酸性能的基础上,在30L发酵罐中系统优化并研究了氮源、维生素水平及溶氧水平对T.glabrata生产丙酮酸的影响.结果表明,以7g/L尿素作为唯一氮源能促进菌体生长,加快葡萄糖消耗和丙酮酸积累;将硫胺素水平由15μg/L提高至18μg/L,菌体干重提高12.5%,生产强度提高21.2%;将通气量调节至2.0L/min时使丙酮酸产量、得率与生产强度分别提高32.8%,11.7%和59%.优化结果在30L发酵罐中得到验证,丙酮酸产量、得率和生产强度分别为84.2g/L,0.72g/g与1.40g/(L·h),比优化前分别提高21.3%,13.2%和12.9%.The production conditons of pyruvate at a pilot scale of 30-L fermentor through increasing environmental adaptability of Torulopsis glabrata were optimized. The fermentation parameters of T. glabrata at 3-L and 30-L fermentors were compared and its mechanism was elucidated. Following the mechanism,the nutrient（nitrogen and thiamine concentration） and environmental（dissolved oxygen） conditions were studied and optimized in 30-L fermentor,the optimum conditions were obtained as followed： 7 g/L urea,18 μg/L thiamine and 2.0 L/min aeration rate. With the conditions,the titer,yield and productivity of pyruvate were achieved at 84.2 g/L,0.72 g/g and 1.40 g/（L·h） ,respectively,which were 21.3%,13.2% and 12.9% higher than the corresponding values of the control.

关 键 词：光滑球拟酵母 环境适应性 丙酮酸 发酵优化 

分 类 号：TQ921[轻工技术与工程—发酵工程]