Influence of Conditions on Reuterin Accumulation by the Resting Cell Biotransformation Process  

作　　者：陈国 杨道茂 肖雅琴 陈宏文 

机构地区：[1]Department of Bioengineering and Biotechnology, Huaqiao University, Xiamen 361021, China

出　　处：《Chinese Journal of Chemical Engineering》2011年第6期1023-1027,共5页中国化学工程学报（英文版）

基　　金：Supported by the National-Natural Science Foundation of China （20906035） and the Fundamental Research Funds for the Central Universities （JB-JX 1002）.

摘　　要：3-Hydroxypropionaldehyde （3-HPA） is a potential valuable chemical and new broad-spectrum antim-icrobial substance. In order to improve the conversion of 3-HPA/glycerol, our work studied the two-step process from glycerol to 3-HPA, and investigated the influence of cell harvest time, glycerol concentration, biomass con-centration, pH and temperature on the production of 3-HPA by Lactobacillus reuteri CG001, respectively. The re-sults showed that molar conversion yield of 3-HPA/glycerol reached 97.9% under the condition that 200 mmol·L-1 glycerol was converted by 25.3 g·L-1 resting cell for 1 h at 30 ℃. The cells could not be reused directly because the L. reuteri almost lost its bioconversion activity completely, but the ability of glycerol conversion could gradually recover if the fresh medium was added to the deactivated cell for 4 h.3-Hydroxypropionaldehyde (3-HPA ) 是一种潜在的珍贵化学、新的用途广泛的抗菌剂物质。以便改进 3-HPA/glycerol 的变换，我们的工作从甘油学习了二拍子的圆舞过程到 3-HPA，并且分别地由乳杆菌 reuteri CG001 在 3-HPA 的生产上调查了房间收获时间，甘油集中，生物资源集中， pH 和温度的影响。结果证明 3-HPA/glycerol 的臼齿的变换产量在条件下面到达了 97.9% 那 200 mmol 摤敬吗？

关 键 词：Lactobacillus reuteri 3-HYDROXYPROPIONALDEHYDE REUTERIN bioconversion 

分 类 号：TQ465[化学工程—制药化工] TQ920.1[轻工技术与工程—发酵工程]