福寿螺β-葡萄糖苷酶在脲溶液中失活动力学研究  

Kinetics of Inactivation of β-glucosidase from Ampullarium crossean in Urea Solutions

作　　者：陈清西[1] 石艳[1] 张吉吉 黄建设 庄总来[1] 

机构地区：[1]厦门大学生物学系,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2000年第1期102-106,共5页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金!(C97005)

摘　　要：研究福寿螺β－葡萄糖苷酶在脲溶液中变性作用，测定酶在脲溶液中的失活动力学，测定游离酶（Ｅ）和酶－底物络合物（ＥＳ）的脲失活的微观速度常数，并加以比较，表明底物存在对于酶被脲的失活具有明显的保护作用．The inactivation of β glucosidase from Ampullarium crossean has been studied using the kinetic method of the substrate reaction during denaturation in urea solutions.The microscopic rate constants of inactivation for the free enzyme and the enzyme substrate complex were determined. Comparison of these rate constants indicates that the presence of substrate offers marked protection of this enzyme against inactivation by urea.

关 键 词：福寿螺 Β-葡萄糖苷酶 失活动力学 变性 

分 类 号：Q556.2[生物学—生物化学] Q959.212.3