中心复合实验优化嗜单宁管囊酵母发酵生产乙醇的研究(Ⅰ)  

STUDY OF CENTRAL COMPOSITE DESIGN IN OPTIMIZATION OF Pachysolen tannophilus FERMENTATION TO PRODUCE ETHANOL

作　　者：程荷芳[1,2,3] 卫民[1,2,3] 蒋剑春[1,2,3] 张宁[1,2,3] 

机构地区：[1]中国林业科学研究院林产化学工业研究所 [2]生物质化学利用国家重点实验室 [3]国家林业局林产化学工程重点开放性实验室,南京210042

出　　处：《太阳能学报》2012年第1期173-178,共6页Acta Energiae Solaris Sinica

基　　金：引进国际先进林业科学技术创新项目(2006-4-C06);林业公益性行业专项-纤维素类生物质热化学-生物复合转化机理研究(200904055)

摘　　要：采用四因素三水平中心复合实验法优化嗜单宁管囊酵母(Pachysolen tannophilus)发酵生产乙醇的条件,根据实验数据拟合建立关于乙醇浓度随发酵时间、接种量、转速、发酵温度等因素变化的数学模型。根据该模型进行工艺参数的优化,以乙醇浓度为指标,实验所得的嗜单宁管囊酵母发酵生产乙醇的优化工艺条件为:发酵时间68h,接种量6%,转速120r/min,发酵温度32℃。该条件下乙醇产量为20.58g/L,残葡萄糖浓度为0.119g/L。对模型的有效性进行检验。The ethanol production by Pachysolen tannophilus fermentation was optimized by 4-factors and 3-levels central composite design. Effects of fermentation time, inoculation, rotation speed and fermentation temperature on ethanol production were investigated and made a mathematical model according to the results. By investigating the ethanol concentration, the optimized technological parameters were as follows ： fermentation time 68 hours, inocula- tion 6%, rotation speed 120r/min and fermentation temperature 32℃. Under these conditions, the ethanol concen- tration was up to 20.58g/L and 0.119g/L rest glucose concentration. The adequacy of the model equation for pre- dicting the optimum response values was verified effectively by the validation.

关 键 词：嗜单宁管囊酵母 发酵 乙醇 中心复合实验 

分 类 号：TQ926.4[轻工技术与工程—发酵工程]