冰硼散-壳聚糖膜的生物相容性研究  

Biocompatibility of Bingpeng Powder-Chitosan Composite Biomaterials

作　　者：顾晓霞[1] 林军[2] 范丽[2] 董陈[2] 

机构地区：[1]复旦大学附属金山医院口腔科,上海200540 [2]浙江大学附属第一医院口腔科,浙江杭州310003

出　　处：《中国临床医学》2011年第6期742-745,共4页Chinese Journal of Clinical Medicine

基　　金：浙江省自然科学基金资助项目(编号:Y2080338)

摘　　要：目的:探讨冰硼散-壳聚糖膜的生物相容性,为引导性牙周组织再生术提供性能优越的膜性材料。方法:制备冰硼散-壳聚糖膜,用MTS法检测其对MG-63细胞的毒性作用,研究设计分为3个组:冰硼散-壳聚糖膜组、壳聚糖膜组、空白对照组,以24 h、48 h、72 h、96 h为检测时间点。扫描电镜观察在冰硼散-壳聚糖膜和壳聚糖膜上培养1 d、3 d、5 d后的MG-63细胞形态。结果:冰硼散-壳聚糖膜组的细胞增殖与壳聚糖膜组和空白对照组无显著差异(P>0.05),冰硼散-壳聚糖膜和壳聚糖膜在各时间点的细胞毒性为1级。电镜观察结果显示各时间点复合膜和壳聚糖膜上的细胞均生长良好,形态无异常。结论:冰硼散-壳聚糖膜具有较好的生物相容性,有可能成为应用前景良好的引导牙周组织再生膜性材料。Objective：To investigate biocompatibility of the Bingpeng powder-chitosan with human osteosarcoma cells in vitro. Methods：The Bingpeng powder-chitosan was preparaed with osmosis method.Human osteosarcoma cells were cultured on Bingpeng powder-chitosan,chitosan and blank control respectively.24,48,72,96 hours after set up,the proliferation of the cells was examined by MTS method.1,3,5 days after set up,the morphology was observed with scanning electron microscope （SEM）.Results：The proliferation of the cells performed no significant difference between Bingpeng powder-chitosan and chitosan （P〉0.05）.There was also no significant difference between Bingpeng powder-chitosan and blank control group.Cytotoxical grades of Bingpeng powder-chitosan and chitosan were grade 1.SEM demonstrated that the growth and morphology of cells was normal on Bingpeng powder-chitosan and chitosan.Conclusions：Bingpeng powder-chitosan has good biocompatibility without cytotoxic,which may be used as a perfect material for guided tissue regeneration.

关 键 词：冰硼散 壳聚糖 细胞毒性 扫描电镜 

分 类 号：R781[医药卫生—口腔医学]