MTT法检测蛙皮活性多肽促进小鼠淋巴细胞增殖作用  

Detection of effect of Rana Chensinensis skin's active polypeptide on stimulating proliferation of lymphocytes by MTT

作　　者：巢警殳[1] 张岚[2] 杜娟[2] 葛红娟[2] 周涌[2] 

机构地区：[1]吉林大学公共卫生学院,吉林长春130021 [2]吉林医药学院公共卫生系,吉林吉林132013

出　　处：《吉林医药学院学报》2011年第1期15-17,共3页Journal of Jilin Medical University

摘　　要：目的研究林蛙皮活性多肽对小鼠淋巴细胞增殖作用。方法利用溴化四唑蓝法测定TF等促进小鼠脾淋巴细胞的增殖活性。结果实验组细胞免疫活性明显高于对照组(P<0.05)。结论林蛙皮活性多肽对培养的小鼠淋巴细胞具有明显的增殖作用,为今后林蛙皮的开发和利用提供了广阔的前景。Objective To study effect of active polypeptide in Rana Chensinensis skin on rat＇s proliferation of lymphocytes.Methods MTT method was adopted to test proliferative activity of TF which could stimulate proliferation of murine splenic lymphocyte.Results Immunocompetence in the experimental group was obviously higher than that in the control group（P0.05）.Conclusion Active polypeptide in Rana chensinensis skin had an obvious effect on cultivating lymphocytes of rats,which creat broad prospects for future exploitation and use of Rana chensinensis skin.

关 键 词：林蛙皮 活性多肽 促淋巴细胞 增殖作用 

分 类 号：R151.2[医药卫生—营养与食品卫生学]