Study on Rapid Propagation Medium of Atractylodes Lances by Orthogonal Design  

应用正交设计优化南苍术快速繁殖培养基的研究(英文)

作　　者：李文[1] 谢烨明[1] 罗翠平[1] 李金华[1] 曾万勇[1] 

机构地区：[1]武汉工业学院生物与制药工程学院,湖北武汉430023

出　　处：《Agricultural Science & Technology》2012年第1期49-52,共4页农业科学与技术（英文版）

基　　金：Supported by Excellent Young and Middle-aged Scholars Program of Education Bureau of Hubei Province(Q20101707);Innovation Activity Project of College Student in Wuhan Polytechnic University~~

摘　　要：[Objective] The aim was to protect wild resource of Atractylodes lances and to provide technical guidance for industrialization of Atractylodes lances. [Method] The test-tube seedlings were produced from seeds of Atractylodis Lances. At the same time, the effects of 6-BA, NAA and KT on the proliferation of aseptic seedling was studied through the orthogonal test. Effect of NAA concentration on the rooting of aseptic seedling was studied through nine types of light substrate groups, which consist of peat soil, fine sand and cotton seed hull, and their effects on Atractylodes lances growth were studied on with orthogonal design. [Result] The culture medium suitable for the proliferation of the Atractylodes lances tube seedling was MS＋6-BA 1.0 mg/L＋NAA 0.4 mg/L＋KT 0,4 mg/L. And the medium suitable for rooting was 1/2MS＋NAA 0.5 mg/L. The optimal ratio of soil, riversand and cotton seed hull was 3：3：2. [Conclusion] The research selected optimum culture medium, rooting medium and ratio of substrate, which provided technical guidance for laboratorial preservation of germplasm resource of Atractylodes lances and industrialization of its seedling production.[目的]为保护野生苍术资源,并为南苍术工厂化生产提供技术指导。[方法]以南苍术(Atractylodes lancea(Thunb.)DC)种子为材料得到无菌试管苗,应用正交试验法L(934)研究6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)、激动素(KT)及其配比对南苍术无菌苗增殖的影响;NAA浓度对南苍术苗生根的影响;以泥土、河沙、棉籽壳为材料,采用正交试验方法设计9种轻型基质配方,研究其对南苍术生长的影响。[结果]适合南苍术试管苗增殖的培养基为MS+6RR鄄BA2.0mg/L+NAA0.4mg/L+KT0.4mg/L;适合南苍术生根的培养基为1/2MS+NAA0.5mg/L;最适基质配比为泥土:河沙:棉籽壳(体积比)=3:3:2。[结论]筛选出了南苍术增殖培养,生根的最佳配方,基质的最佳配比,为苍术资源的实验室保存和种苗工厂化生产提供了技术依据。

关 键 词：Atractylodes lance（Thunb.）DC. Orthogonal Design HORMONE SUBSTRATE 

分 类 号：S567.211[农业科学—中草药栽培]