Stathmin在上皮性卵巢癌组织中的表达及意义  

The expression and significance of Stathmin in epithelial ovarian cancer

作　　者：石英[1] 翁艳洁[1] 周文娟[1] 周婷[1] 陈刚[1] 王常玉[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,湖北武汉430030

出　　处：《中国癌症杂志》2011年第8期581-584,共4页China Oncology

基　　金：国家自然科学基金(No:30973184)

摘　　要：背景与目的:Stathmin是一种重要的微管调节蛋白,参与多种肿瘤的发生、发展。本研究旨在验证并探讨Stathmin在上皮性卵巢癌中的表达及意义。方法:利用免疫组化和逆转录聚合酶链反应(RT-PCR)方法检测22例卵巢正常上皮组织,16例良性肿瘤和50例上皮性卵巢癌组织中Stathmin的表达。结果:上皮性卵巢癌中Stathmin mRNA的表达明显高于卵巢良性肿瘤(P<0.05)和正常卵巢上皮(P<0.05)。免疫组化方法检测显示,Stathmin蛋白主要定位在细胞的细胞质,且上皮性卵巢癌中Stathmin蛋白阳性率明显高于卵巢良性肿瘤和正常卵巢上皮(74.00%vs 31.25%、9.09%,P<0.01),而卵巢良性肿瘤与正常卵巢上皮相比,差异无统计学意义(P>0.05)。进一步利用χ2检验统计分析得出,Stathmin蛋白与卵巢癌的临床分期、病理分级、淋巴转移相关(P<0.05),而与年龄、病理类型及腹水量无相关性(P>0.05)。结论:Stathmin在上皮性卵巢癌中高表达,可能与卵巢癌的发生、发展相关。Background and purpose： Stathmin is identified as a kind of important microtubules regulatory protein and participates in multiple tumorigenesis. In this study, we investigated the expression and significance of Stathmin in epithelial ovarian cancer. Methods： The expression levels of Stathmin in 22 cases of normal ovarian tissues, 16 cases of benign ovarian tumors and 50 cases of epithelial ovarian cancers were examined by RT-PCR and immunohistochemical staining. Results： The Stathmin mRNA expression in ovarian epithelial cancer was higher than that in normal and benign tumor tissues（P〈0.05）. The immunohistochemical analysis demonstrated that Stathmin protein was mainly located in the cytoplasm and the positive rate of Stathmin in epithelial ovarian cancers group was higher than that in benign ovarian tumor and ovarian cancer group （74.00% vs 31.25% and 9.09%, P〈0.01）. The difference between normal ovarian tissue group and benign ovarian tumor group had no significance （P〉0.05）. X2 analysis was utilized to show that the expression of Stathmin was related to clinical stages, histological grade and lymph node metastasis （P〈0.05）, but not age, pathologic types and the amount of ascites （P〉0.05）. Conclusion： Stathmin overexpression may correlate with the occurrence and progression of epithelial ovarian cancer.

关 键 词：卵巢癌 STATHMIN 免疫组化 逆转录聚合酶链反应 

分 类 号：R730.261[医药卫生—肿瘤] R737.31[医药卫生—临床医学]