亮氨酸对血糖的影响及其相关机制研究  

Effects of leucine on blood glucose and its mechanism

作　　者：王觐[1] 薛长勇 徐庆[1] 张月红[1] 刘英华[1] 张永[1] 杨雪艳[1] 宫雪[1] 

机构地区：[1]解放军总医院营养科,北京100853

出　　处：《军医进修学院学报》2012年第2期132-134,共3页Academic Journal of Pla Postgraduate Medical School

摘　　要：目的探讨亮氨酸对血糖水平的影响及其机制。方法将45只昆明小鼠随机分为3组,每组15只。亮氨酸低剂量组给予2.0g/kg葡萄糖和0.7g/kg亮氨酸混合液,亮氨酸高剂量组给予2.0g/kg葡萄糖和2.0g/kg亮氨酸混合液,对照组均仅给予2.0g/kg葡萄糖,灌胃后0.5h、1h、2h检测血糖。为排除亮氨酸本身对肠道葡萄糖吸收的影响给予淀粉糖耐量实验,亮氨酸组给予2.0g/kg淀粉和2.0g/kg亮氨酸混合液,对照组给予2.0g/kg淀粉,灌胃后0.5h、1h、2h检测血糖。在灌胃葡萄糖和亮氨酸后0.5h眼眶取血,ELISA方法测定血样胰岛素和胰高血糖素样肽-1(GLP-1)。结果亮氨酸低剂量组0.5h、1h血糖及血糖曲线下面积(AUC)显著低于对照组,亮氨酸高剂量组在0.5h、1h血糖及AUC显著低于对照组,亮氨酸低剂量组与高剂量组0.5h血糖有显著差异。亮氨酸组灌胃给予淀粉0.5h、1h血糖及AUC显著低于对照组。亮氨酸高剂量组胰岛素和GLP-1水平均显著高于对照组(P<0.05),而亮氨酸低剂量组上述指标较对照组无显著性差异。结论亮氨酸通过促进胰岛素和GLP-1分泌来降低血糖。Objective To study the effect of leucine on blood glucose and its mechanism. Methods Forty-five Kunming mice were divided into low-leucine group, high-leucine group, and control group（15 in each group）. Mice in low-leucine group were orally given a mixture of leucine（0.7g/kg） and glucose（2.0g/kg）, those in high-leucine group were orally given a mixture of leucine（2.0g/kg） and glucose（2.0g/kg）, and those in control group were orally given glucose（2.0g/kg） only. Blood glucose level was measured at 0.5, 1 and 2h, respectively, after ingestion of leucine and glucose. Glucose tolerance was tested after oral intake of starch to exclude the influence of oral intake of leucine on glucose absorption in the gastrointestinal tract. Blood glucose level was measured at 0.5, 1 and 2h after ingestion of leucine and starch. In another experiment, mice were killed at 0.5 after oral administration of glucose and leucine. Blood insulin and glucagon like peptides-l（GLP-1） levels were measured by ELISA. Results The levels of blood glucose at 0.5h and lh after oral intake of leucine, glucose and AUC were significantly lower in low-leucine group and in high-leucine group than those in control group, respectively. The levels of blood glucose at 0.5h after oral intake of leucine, glucose and AUC were significantly lower in high-leucine group than those in low-leucine group; The levels of blood glucose at 0.5h, lh and 2h after oral intake of leucine, starch and AUC were significantly lower than those in control group; The concentration of insulin and GLP-1 increased significantly in high-leucine group than those in control group, but no significant changes were noted between low-leucine and control group. Conclusion Leucine decreased blood glucose by increasing insulin and GLP-1 secretion.

关 键 词：亮氨酸 血糖 胰岛素 胰高血糖素样肽-1 

分 类 号：R151[医药卫生—营养与食品卫生学]