Nitroglycerine effects on portal vein mechanics and oxidative stress in portal hypertension  

作　　者：Andreja Vujanac Vladimir Jakovljevic Dusica Djordjevic Vladimir Zivkovic Mirjana Stojkovic Dragan Celikovic Nebojsa Andjelkovic Aleksandra Jurisic Skevin Dragan Djuric 

机构地区：[1]Department of Physiology,Faculty of Medicine,University of Kragujevac [2]Department of Gastroenterology and Echosonography Unit,Clinical Centre of Belgrade [3]Internal Clinic,Clinical Centre "Kragujevac",Faculty of Medicine,University of Kragujevac [4]Department of Physical Medicine and Rehabilitation,Faculty of Medicine,University of Kragujevac [5]Institute of Medical Physiology,Belgrade University School of Medicine

出　　处：《World Journal of Gastroenterology》2012年第4期331-339,共9页世界胃肠病学杂志（英文版）

基　　金：Supported by The Grant from the Ministry of Science and Technical Development of the Republic of Serbia,No.175043

摘　　要：AIM:Тo examine the effects of nitroglycerine on portal vein haemodynamics and oxidative stress in patients with portal hypertension.METHODS:Thirty healthy controls and 39 patients with clinically verified portal hypertension and increasedvascular resistance participated in the study.Liver di-ameters,portal diameters and portal flow velocities were recorded using color flow imaging/pulsed Doppler detection.Cross-section area,portal flow and index of vascular resistance were calculated.In collected blood samples,superoxide anion radical (O 2-),hydrogen per-oxide (H 2 O 2),index of lipid peroxidation (measured as TBARS) and nitric oxide (NO) as a marker of endothelial response (measured as nitrite-NO 2-) were determined.Time-dependent analysis was performed at basal state and in 10th and 15th min after nitroglycerine (sublingual 0.5 mg) administration.RESULTS:Oxidative stress parameters changed sig-nificantly during the study.H 2 O 2 decreased at the end of study,probably via O 2-mediated disassembling in Haber Weiss and Fenton reaction;O 2-increased signifi-cantly probably due to increased diameter and tension and decreased shear rate level.Consequently O 2-and H 2 O 2 degradation products,like hydroxyl radical,initi-ated lipid peroxidation.Increased blood flow was to some extent lower in patients than in controls due to double paradoxes,flow velocity decreased,shear rate decreased significantly indicating non Newtonian char-acteristics of portal blood flow.CONCLUSION:This pilot study could be a starting point for further investigation and possible implemen-tation of some antioxidants in the treatment of portal hypertension.AIM：Тo examine the effects of nitroglycerine on portal vein haemodynamics and oxidative stress in patients with portal hypertension.METHODS：Thirty healthy controls and 39 patients with clinically verified portal hypertension and increasedvascular resistance participated in the study.Liver di-ameters,portal diameters and portal flow velocities were recorded using color flow imaging/pulsed Doppler detection.Cross-section area,portal flow and index of vascular resistance were calculated.In collected blood samples,superoxide anion radical （O 2-）,hydrogen per-oxide （H 2 O 2）,index of lipid peroxidation （measured as TBARS） and nitric oxide （NO） as a marker of endothelial response （measured as nitrite-NO 2-） were determined.Time-dependent analysis was performed at basal state and in 10th and 15th min after nitroglycerine （sublingual 0.5 mg） administration.RESULTS：Oxidative stress parameters changed sig-nificantly during the study.H 2 O 2 decreased at the end of study,probably via O 2-mediated disassembling in Haber Weiss and Fenton reaction;O 2-increased signifi-cantly probably due to increased diameter and tension and decreased shear rate level.Consequently O 2-and H 2 O 2 degradation products,like hydroxyl radical,initi-ated lipid peroxidation.Increased blood flow was to some extent lower in patients than in controls due to double paradoxes,flow velocity decreased,shear rate decreased significantly indicating non Newtonian char-acteristics of portal blood flow.CONCLUSION：This pilot study could be a starting point for further investigation and possible implemen-tation of some antioxidants in the treatment of portal hypertension.

关 键 词：Portal hypertension ENDOTHELIUM NITROGLYCERINE Oxidative stress Portal vein haemodynamics 

分 类 号：R575.2[医药卫生—消化系统]