酶解菲牛蛭制备抗凝血肽的工艺优化  

Optimization of Enzymatic Hydrolysis of Poecilobdella manillensis Lesson for Preparing of Ancoagulant Peptide

作　　者：钟超[1] 吴晖[1] 赖富饶[1] 

机构地区：[1]华南理工大学食品安全与检测研究中心,广东广州510640

出　　处：《现代食品科技》2012年第2期164-167,155,共5页Modern Food Science and Technology

基　　金：新世纪优秀人才支持计划项目(NCET-060746)

摘　　要：以菲牛蛭为原料,采用Alcalase 2.4 L碱性蛋白酶酶解菲牛蛭制备抗凝血肽,通过筛选显著单因素和设计正交试验,并以抗凝血活性为指标,确定酶解的最佳反应条件为:酶解温度55℃、酶解时间2 h、底物浓度12%、加酶量3%、pH 8.5,在该条件下所制备的抗凝血肽的抗凝血活性可达702AT-U/g。In order to obtain high activity ancoagulant peptide from Poecilobdella manillensis Lesson, an enzymolysis method was established through sequential steps of screening single-factor and designing orthogonal test. By selecting ancoagulant activity as index, the optimum enzymolysis reaction conditions were determined as enzymolysis temperature 55℃, enzymolysis temperature 2h, substrate concentration 12%, enzyme dosage 3% and pHS.5. The ancoagulant activity could reach 702AT-U/g under the optimized conditions.

关 键 词：菲牛蛭 抗凝血活性 ALCALASE 2.4L 抗凝血肽 

分 类 号：TS254.1[轻工技术与工程—水产品加工及贮藏工程]