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作 者:张鹤[1] 佟心洁[1] 庞春生[1] 谢土均[1]
机构地区:[1]华南理工大学轻工与食品学院,广东广州510640
出 处:《现代食品科技》2012年第2期182-186,共5页Modern Food Science and Technology
基 金:国家重点基础研究发展计划(973计划)项目(2010CB732201);华南理工大学轻工与食品实验教学示范中心建设项目资助
摘 要:本文以玉米秸秆活性氧固体碱蒸煮浆(木素脱除率82.1%)为原料,用国产纤维素酶(酶活为13.94 FPU/100 mL)进行酶解试验,探索酶解最佳工艺条件。结果显示:底物固液比为0.47%(m/V),酶解pH值为4.70,酶解温度为50℃,酶解时间为72 h时酶解液还原糖含量最大,即糖得率最大,达59.41%。本研究也进行玉米秸秆经不同方法预处理后的酶解实验,结果显示其纤维素酶解效果顺序为:活性氧固体碱蒸煮浆>硫酸盐浆>机械粉碎>未预处理玉米秸秆。The objective of this study was to explore technique of enzymatic hydrolysis of com stalk pulp (removal rate of lignin of 82.1%) from active oxygen cooking with a solid alkali with Chinese cellulase (enzyme activity: 13.94 FPU/10Oml). The results showed that the optimum process conditions were the following: enzyme-substrate ratio of 0.47% (m/F), pH value of 4.70, temperature of 50℃ and enzymatic reaction time of 72 h. The highest yield of sugar was 59.41% under the optimum process conditions. The paper also presented the different enzymolysis of corn stalk pretmated by different methods, and the order of the effect of enzymolysis was as follows: corn stalk pulp from active oxygen cooking with a solid base 〉 corn stalk pulp from alkaline cooking 〉〉 corn stalk pretreated by mechanical crushing 〉 raw corn stalk.
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