适合SDS-PAGE分析的黑根霉菌丝蛋白质提取方法  

Protein extraction methods suitable for SDS-PAGE analysis from hyphae of Rhizopus nigricans

作　　者：孙晓光[1] 路福平[1] 别松涛[1] 刘晓[1] 谢进霞[1] 

机构地区：[1]天津科技大学生物工程学院,天津市工业微生物重点实验室,天津300457

出　　处：《工业微生物》2012年第1期17-22,共6页Industrial Microbiology

基　　金：国家自然基金(20906071);天津市科技支撑计划项目(10ZCKFSH00600)

摘　　要：为制备适合SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)分析的黑根霉菌丝蛋白样品,采用不同的提取方法对黑根霉的菌丝进行蛋白质提取。结果表明:黑根霉菌丝经低温冷冻过夜后,直接用0.2 mol/L NaOH处理10 min,再加入上样缓冲液沸水浴,这一方法能快速、方便、有效的提取蛋白质。利用该方法比较了黑根霉与黑根霉甲苯耐受菌菌丝蛋白组成上的差异。In order to develop an efficient protein extraction method suitable for SDS-PAGE analysis, different extraction protocols were used for protein extraction from the hyphae of Rhizopus nigricans. The result showed that the hyphae of Rhizopus nigricans, freezed overnight, was subjected to 0.2 mol/L sodium hydroxide for 10 rain and then boiled for 5 min in a standard electrophoresis loading buffer could rapidly, easily and effectively extract protein. Compositive changes of mecelium proteins in Rhizopus nigricans and the mutant of Rhizopus nigricans resistant to toluene were analyzed by this method.

关 键 词：黑根霉菌丝 SDS-PAGE 蛋白质提取 

分 类 号：TQ931[化学工程]