高效液相色谱法测定西兰花中萝卜硫素的研究  

Study on determination method of sulforaphane in broccoli by HPLC

作　　者：顾颖娟[1] 张亮[1] 邹宇[1] 陈志刚[1] 韩永斌[1] 顾振新[1] 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品工业科技》2012年第4期80-82,85,共4页Science and Technology of Food Industry

基　　金：江苏省科技支撑计划项目(BE2006309)

摘　　要：建立了采用高效液相色谱法(HPLC)测定西兰花种子及芽苗中萝卜硫素的方法。具体方法为:采用反相XDB-C18色谱柱分离,乙腈-水梯度(10%-60%-100%,0-25-30min)洗脱,流速0.6mL/min,检测波长254nm,进样量50μL。萝卜硫素测定结果的相对标准偏差小于1%,平均回收率达到97.14%。该方法具有较好的分离效果和较高的稳定性,适用于西兰花中萝卜硫素含量的测定。A high performance liquid chromatograph（HPLC） method had been set up for determination of content of sulforaphane in broccoli seeds and buds.The chromatographic separation was achieved on reversed XDB-C18 using acetonitrile-water as the mobile phase（10%-60%-100%,0-25-30min） at a flow rate of 0.6mL/min.The detector wavelength was set at 254nm and the injection volume used was 50μL.The relative standard deviation of sulforaphane content determinated was less than 1% and the average recovery was 97.14%.This method showed good separation effect and high stability,and could be used for determining the content of sulforaphane in broccoli.

关 键 词：西兰花 高效液相色谱法 萝卜硫素 测定 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]