果胶酶辅助提取裙带菜孢子叶多糖的工艺条件优化  

Optimization on pectase-assistant extracting technological conditions of polysaccharide from sporophyll of Undaria pinnatifida by response surface methodology

作　　者：余洋定[1,2,3] 启航[1,2,3] 李冬梅[1,2,3] 何尧斌[1,2,3] 毕景然[1,2,3] 

机构地区：[1]大连工业大学食品学院,辽宁大连116034 [2]海洋食品教育部工程研究中心,辽宁大连116034 [3]辽宁省海洋食品科学与技术重点实验室,辽宁大连116034

出　　处：《食品与机械》2012年第1期175-177,共3页Food and Machinery

基　　金：星火计划(编号:2010GA651007);农转资金项目(编号:2009B00099)

摘　　要：为优化裙带菜孢子叶多糖的提取工艺,利用响应面中的Box-Behnken设计优化果胶酶辅助提取裙带菜孢子叶多糖的工艺条件。以多糖得率为评价指标,最终确定果胶酶辅助提取裙带菜孢子叶多糖工艺条件为酶加量0.65%、液料比59∶1(V∶m),pH值3.4。该条件下,裙带菜孢子叶多糖得率为4.79%。To optimize the extracting technology of polysaccharide from sporophyll of Undaria pinnatifida,the technological condition of pectase-assistant extracting polysaccharide from sporophyll of Undaria pinnatifida was optimized by response surface methodology（RSM）.Polysaccharide extraction yield was as evaluated index and final optimal technological conditions of pectase-assistant extracting polysaccharide were as follows： the amount of pectase was 0.65%,the ratio of water and material was 59：1（V∶m） and pH was 3.4,respectively.At this condition,polysaccharide extracting yield from sporophyll of Undaria pinnatifida was 4.79%.

关 键 词：裙带菜 孢子叶 多糖 果胶酶 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]