质谱法测定4种真菌毒素与载体蛋白偶联物的结合比  

Determination of Coupling Ratios of Four Mycotoxins-Carrier Protein Conjugates by Mass Spectrometry

作　　者：王莹[1] 王希春[1] 金福源 何成华[1] 郑浩[1] 张海彬[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]汾湖动物防疫站,江苏吴江215211

出　　处：《食品科学》2012年第2期219-223,共5页Food Science

基　　金：国家"863"计划项目(2007AA10Z429)

摘　　要：采用基质辅助激光解吸-电离飞行时间质谱法(matrix-assisted laser desorption/ionization time-of-flight massspectrometry,MALDI-TOFMS)对由3种真菌毒素伏马菌素B1(FB1)、赭曲霉毒素A(OTA)、脱氧雪腐镰刀菌烯醇(DON)与两种载体蛋白卵清白蛋白(OVA)、血清白蛋白(BSA)分别偶联制备得到的4种小分子-载体蛋白偶联物FB1-OVA、FB1-BSA、OTA-BSA、DON-OVA的偶联结合比进行测定,并与紫外吸收光谱法进行比较。基质辅助激光解吸-电离飞行时间质谱法测得偶联物FB1-OVA、FB1-BSA、OTA-BSA、DON-OVA的结合比分别为5:1、11:1、3:1、0;紫外吸收光谱法测得OTA-BSA的结合比为4.4:1,DON-OVA的结合比为3.4:1,无法测定FB1-OVA、FB1-BSA结合比。Three mycotoxins including fumonisin B1（FB1）,ochrotoxin A（OTA） and deoxynivalenol（DON） were coupled with two carrier proteins such as ovalbumin（OVA） and bovine serum albumin（BSA）,respectively.Four small molecule-carrier protein conjugates（FB1-OVA,FB1-BSA,OTA-BSA and DON-OVA） were prepared.The coupling ratios of the conjugates were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOFMS） and ultraviolet（UV） absorption spectroscopy,respectively.The coupling ratios of FB1-OVA,FB1-BSA,OTA-BSA and DON-OVA were 5：1,11：1,3：1 and 0,respectively as determined by MALDI-TOFMS.However,the coupling ratios of OTA-BSA and DON-OVA determined by UV absorption spectroscopy were 4.4：1 and 3.4：1,respectively.The coupling ratios of FB1-OVA and FB1-BSA could not be determined by UV absorption spectroscopy.

关 键 词：基质辅助激光解吸-电离飞行时间质谱法 紫外吸收光谱法 真菌毒素 载体蛋白 偶联物 结合比 

分 类 号：S852.4[农业科学—基础兽医学]