镍与绿茶EGCG对舌鳞癌细胞毒性的影响  

Effect of Interaction of Ni^(2+) and Green Tea Epigallocatechin Gallate on Tongue Carcinoma Cells

作　　者：罗真兰[1] 王海波[1] 苏俭生[1] 

机构地区：[1]同济大学口腔医学院,上海200072

出　　处：《无机材料学报》2012年第2期179-184,共6页Journal of Inorganic Materials

基　　金：国家自然科学基金(30840031;30970726)~~

摘　　要：Ni2+是镍基牙科铸造合金中析出的主要金属离子,是人体的致癌物质.绿茶的主要活性成分表没食子儿茶素没食子酸酯(EGCG)对包括口腔癌在内的人体癌有化学预防作用.本研究采用LC/MS法分析EGCG与Ni2+间的相互作用.MTT法检测EGCG、Ni2+及其相互作用对舌鳞癌细胞生长的影响.LC/MS分析显示,Ni2+与EGCG同时加入时有[EGCG-2Ni2+]和[EGCG-Ni2+]形成.Ni2+和EGCG在一定范围内对舌鳞癌细胞生长的抑制呈时间及浓度依赖性.EGCG与Ni2+共同作用后可明显增强对舌鳞癌细胞的抑制作用,且抑制作用与它们的浓度、加入的顺序及Ni2+与EGCG的比例有关.Ni2＋ is the major ion release from nickel-based dental casting alloy and carcinogens.The major polyphenolic constituent present in green tea,（-）epigallocatechin-3-gallate（EGCG）,shows chemoprevention on human cancer.The aim of this study was to determine the interaction between EGCG and Ni2＋ and the biological effects on tongue squamous cell carcinoma.The interaction of EGCG and Ni2＋ was analyzed by LC/MS.The cell viability was assessed by the MTT reduction assay.The results of LC/MS indicated that [EGCG-2Ni2＋] and [EGCG-Ni2＋] formed when EGCG and Ni2＋ were added simultaneously.Ni2＋ and EGCG both inhibited the proliferation of tongue carcinoma cells in a time-and dose-dependent manner.The cytotoxicity against tongue carcinoma cells was increased when EGCG and Ni2＋ cotreated,and the inhibition effects were extremely dependent on the concentrations of EGCG and Ni2＋,addition order and the ratio of [Ni2＋] to [EGCG].

关 键 词：牙科铸造合金 绿茶 NI2+ EGCG 舌鳞癌细胞 

分 类 号：R783[医药卫生—口腔医学]