牛磺酸对沙土鼠短暂性前脑缺血作用研究  

Protection of Taurine against Transient Forebrain Ischemia in Gerbils

作　　者：赵育梅[1] 孙明[1] 徐超[1] 

机构地区：[1]北京市神经外科研究所神经介质室,北京100050

出　　处：《中国卒中杂志》2012年第2期98-102,共5页Chinese Journal of Stroke

摘　　要：目的观察牛磺酸对沙土鼠短暂性前脑缺血的保护作用。方法结扎沙土鼠双侧颈动脉建立前脑缺血模型,缺血5 min后进行再灌注。在缺血1 h后经静脉给予2.5 mg/kg、5 mg/kg、15 mg/kg及50 mg/kg牛磺酸。再灌注7 d时,对沙土鼠的神经功能行为缺损进行评分,并留取全脑,观察海马CA1区锥体细胞的死亡情况。结果 2.5 mg/kg牛磺酸虽然改善前脑缺血5 min再灌注7 d时沙土鼠的行为,降低海马CA1区锥体细胞的死亡,但与缺血对照组相比,无统计学意义,5 mg/kg、15 mg/kg及50 mg/kg牛磺酸可明显改善前脑缺血沙土鼠的行为缺损(P分别为0.001、0.006和0.037),降低海马CA1区锥体细胞的死亡(P均<0.001),其中,5 mg/kg、15 mg/kg和50 mg/kg牛磺酸组的锥体细胞的死亡低于2.5 mg/kg牛磺酸组(P分别为0.001、0.001和0.005)。结论牛磺酸能以剂量依赖方式降低沙土鼠前脑缺血损伤,提示牛磺酸可能是一种有效的短暂性缺血的大脑保护剂。Objective To investigate the protection of taurine against transient forebrain ischemia in gerbils. Methods The model of transient forebrain ischemia in gerbil was induced through ligating bilateral carotid arteries for 5 min. The dose of 2.5, 5, 15 or 50 mg/kg of taurine was administrated intravenously 1 h after ischemia. The behavior and the neuronal death in hippocampal CAI region were evaluated 7 days after induction of ischemia. Results Treatment with 2.5 mg/kg of taurine improved gerbil behavior and reduced the neuronal death in hippocampal CAI region, although it did not reach statistical significance. Taurine at dose of 5, 15 or 50 mg/kg improved gerbil behavior（P=0.001, 0.006, and 0.037, respectively）, and reduced the neuronal death in hippocampal CAI region significantly（all P=0.0001）. Moreover, the efficacies of 5, 15, and 50 mg/kg of taurine were better than that of 2.5 mg/kg of taurine（P=0.001, 0.001, and 0.005, respectively）. Conclusions Taurine attenuates the brain damage due to transient forebrain ischemia in a dose- dependent manner, suggesting that taurine is an efficacious neuroprotectant.

关 键 词：短暂性脑缺血 沙土鼠 牛磺酸 缺血大脑保护 

分 类 号：R743[医药卫生—神经病学与精神病学]